RecombinantRabMAb

Recombinant Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

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Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
  • Immunocytochemistry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
  • Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10496] to MAT2A + MAT1A
  • Suitable for: Flow Cyt (Intra), WB, ICC
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

Overview

  • Product name

    Anti-MAT2A + MAT1A antibody [EPR10496]
    See all MAT2A+MAT1A primary antibodies

  • Description

    Rabbit monoclonal [EPR10496] to MAT2A + MAT1A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
    Database link: P31153

  • Positive control

    • ICC: HeLa cells; WB: Human MAT1A/2A recombinant protein, HeLa, HepG2, Ramos, 293T, NIH/3T3 and C6 whole cell lysates; Flow Cyt (intra): Ramos and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.
WB
1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC
1/100.
Notes
Flow Cyt (Intra)
1/30.
WB
1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC
1/100.

Images

  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44, 46 kDa why is the actual band size different from the predicted?



    MAT1A: 44kDa ; MAT2A: 46kDa 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Immunocytochemistry - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:100 dilution (2.52 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution

    Lane 1 : Full-length Human MAT1A recombinant protein with His-Tag
    Lane 2 : Full-length Human MAT2A recombinant protein with GST-Tag

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa



    MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house
    MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company

    Blocking and diluting buffer and concentrations: 5%NFDM/TBST

    Exposure time: Lane 1: 1 second
                           Lane 2: 3 seconds

     

     

  • Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)
    Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

References (2)

Publishing research using ab177484? Please let us know so that we can cite the reference in this datasheet.

ab177484 has been referenced in 2 publications.

  • Bai J  et al. Identification of a natural inhibitor of methionine adenosyltransferase 2A regulating one-carbon metabolism in keratinocytes. EBioMedicine N/A:N/A (2018). PubMed: 30591370
  • Miousse IR  et al. Modulation of dietary methionine intake elicits potent, yet distinct, anticancer effects on primary versus metastatic tumors. Carcinogenesis 39:1117-1126 (2018). WB ; Mouse . PubMed: 29939201

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