Recombinant Anti-MDH2 antibody [EPR14882(B)] (ab181873)
![Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)](https://www.abcam.com/ps/products/181/ab181873/Images/ab181873-397685-anti-mdh2-antibody-epr14882-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14882(B)] to MDH2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MDH2 antibody [EPR14882(B)]
See all MDH2 primary antibodies -
Description
Rabbit monoclonal [EPR14882(B)] to MDH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, K562, HepG2 and HeLa cell lysates; Human eyeball tissue lysate. IHC-P: Human infiltrating duct carcinoma of breast and mouse testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14882(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family. -
Post-translational
modificationsAcetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 4191 Human
- Entrez Gene: 17448 Mouse
- Entrez Gene: 81829 Rat
- Omim: 154100 Human
- SwissProt: P40926 Human
- SwissProt: P08249 Mouse
- SwissProt: P04636 Rat
- Unigene: 520967 Human
see all -
Alternative names
- M MDH antibody
- Malate dehydrogenase 2, NAD (mitochondrial) antibody
- Malate dehydrogenase antibody
see all
Images
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Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)All lanes : Anti-MDH2 antibody [EPR14882(B)] (ab181873) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : MDH2 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Human eyeball tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1-4: Merged signal (red and green). Green - ab181873 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.
ab181873 Anti-MDH2 antibody [EPR14882(B)] was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181873 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)](https://www.abcam.com/ps/products/181/ab181873/Images/ab181873-212903-anti-mdh2-antibody-epr14882b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)Immunohistochemical analysis of Human infiltrating duct carcinoma of breast tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)](https://www.abcam.com/ps/products/181/ab181873/Images/ab181873-212902-anti-mdh2-antibody-epr14882b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)Immunohistochemical analysis of mouse testis tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)](https://www.abcam.com/ps/products/181/ab181873/Images/ab181873-212904-anti-mdh2-antibody-epr14882b-western-blot.jpg)
Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)All lanes : Anti-MDH2 antibody [EPR14882(B)] (ab181873) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
![Anti-MDH2 antibody [EPR14882(B)] (ab181873)](https://www.abcam.com/ps/products/181/ab181873/Images/ab181873-5-benefits-of-recombinant-antibodies.png)
Anti-MDH2 antibody [EPR14882(B)] (ab181873)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab181873 has been referenced in 4 publications.
- Yang Y et al. Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. Virol J 18:39 (2021). PubMed: 33602268
- Ji D et al. Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function. Sci Rep 10:20324 (2020). PubMed: 33230189
- Skinner OS et al. Top-down characterization of endogenous protein complexes with native proteomics. Nat Chem Biol 14:36-41 (2018). PubMed: 29131144
- Miikkulainen P et al. HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer Metab 5:5 (2017). PubMed: 28680592
