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  1. Link

    mdh2-antibody-epr14882b-ab181873.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MDH2 antibody [EPR14882(B)] (ab181873)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (4)

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Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
  • Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
  • Anti-MDH2 antibody [EPR14882(B)] (ab181873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14882(B)] to MDH2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
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Anti-NDUFA9 antibody [20C11B11B11] (ab14713)
Knockout
Product image
Human MDH2 knockout HEK-293T cell line (ab266449)
Protein
Product image
Recombinant human MDH2 protein (ab99238)

View more associated products

Overview

  • Product name

    Anti-MDH2 antibody [EPR14882(B)]
    See all MDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14882(B)] to MDH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, Jurkat, K562, HepG2 and HeLa cell lysates; Human eyeball tissue lysate. IHC-P: Human infiltrating duct carcinoma of breast and mouse testis tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14882(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-MDH2 antibody [EPR14882(B)] - BSA and Azide free (ab250541)
  • Assay kits

    • Malate Dehydrogenase 2 (MDH2) Activity Assay (ab119693)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MDH2 knockout HEK-293T cell line (ab266449)
  • KO cell lysates

    • Human MDH2 knockout HEK-293T cell lysate (ab257533)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human MDH2 protein (ab99238)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications

    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P40926 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4191 Human
    • Entrez Gene: 17448 Mouse
    • Entrez Gene: 81829 Rat
    • Omim: 154100 Human
    • SwissProt: P40926 Human
    • SwissProt: P08249 Mouse
    • SwissProt: P04636 Rat
    • Unigene: 520967 Human
    • Unigene: 297096 Mouse
    • Unigene: 1011 Rat
    see all
  • Alternative names

    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Images

  • Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    All lanes : Anti-MDH2 antibody [EPR14882(B)] (ab181873) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : MDH2 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Human eyeball tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab181873 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab181873 Anti-MDH2 antibody [EPR14882(B)]  was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181873 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)

    Immunohistochemical analysis of Human infiltrating duct carcinoma of breast tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14882(B)] (ab181873)

    Immunohistochemical analysis of mouse testis tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    Western blot - Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    All lanes : Anti-MDH2 antibody [EPR14882(B)] (ab181873) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Anti-MDH2 antibody [EPR14882(B)] (ab181873)
    Anti-MDH2 antibody [EPR14882(B)] (ab181873)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (4)

Publishing research using ab181873? Please let us know so that we can cite the reference in this datasheet.

ab181873 has been referenced in 4 publications.

  • Yang Y  et al. Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. Virol J 18:39 (2021). PubMed: 33602268
  • Ji D  et al. Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function. Sci Rep 10:20324 (2020). PubMed: 33230189
  • Skinner OS  et al. Top-down characterization of endogenous protein complexes with native proteomics. Nat Chem Biol 14:36-41 (2018). PubMed: 29131144
  • Miikkulainen P  et al. HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer Metab 5:5 (2017). PubMed: 28680592

Customer reviews and Q&As

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Western blot abreview for Anti-MDH2 antibody [EPR14882(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasmic Extract)
Specification
Muscle Sarcoplasmic Extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Shannon Cruzen

Verified customer

Submitted Feb 18 2015

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