RecombinantRabMAb

Recombinant Anti-Mesothelin antibody [SP74] (ab93620)

Reviews (2)Q&A (5)References (3)
Western blot - Anti-Mesothelin antibody [SP74] (ab93620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
  • Anti-Mesothelin antibody [SP74] (ab93620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP74] to Mesothelin
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Mesothelin antibody [SP74]
    See all Mesothelin primary antibodies

  • Description

    Rabbit monoclonal [SP74] to Mesothelin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide within Human Mesothelin aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13421

  • Positive control

    • IHC-P: Human Mesothelioma tissue. WB: BxPC-3, HeLa, PC3 and A549 cell lysate.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P (2)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

  • Sequence similarities

    Belongs to the mesothelin family.

  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CAK 1 antibody
      • CAK1 antibody
      • CAK1 antigen antibody
      • cleaved form antibody
      • Megakaryocyte potentiating factor antibody
      • Mesothelin antibody
      • Mesothelin isoform 1 precursor antibody
      • MPF antibody
      • Msln antibody
      • MSLN_HUMAN antibody
      • Pre pro megakaryocyte potentiating factor antibody
      • Pre-pro-megakaryocyte-potentiating factor antibody
      • SMR antibody
      • SMRP antibody
      • Soluble MPF mesothelin related protein antibody
      see all

    Images

    • Western blot - Anti-Mesothelin antibody [SP74] (ab93620)
      Western blot - Anti-Mesothelin antibody [SP74] (ab93620)
      All lanes : Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
      Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate
      Lane 3 : PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate
      Lane 4 : A549 (Human lung carcinoma epithelial cell) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

      Predicted band size: 69 kDa
      Observed band size: 49,69 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer and concentration: 5% NFDM /TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)

      Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)

    • Anti-Mesothelin antibody [SP74] (ab93620)
      Anti-Mesothelin antibody [SP74] (ab93620)

    References (3)

    Publishing research using ab93620? Please let us know so that we can cite the reference in this datasheet.

    ab93620 has been referenced in 3 publications.

    • Kim H  et al. Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma. Medicine (Baltimore) 98:e16207 (2019). PubMed: 31261569
    • Smith TT  et al. Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest 127:2176-2191 (2017). PubMed: 28436934
    • Sopha SC  et al. Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma. Int J Clin Exp Pathol 6:2476-86 (2013). PubMed: 24228110

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mesothelin antibody [SP74]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (mesothelioma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica BOND ER2
    Permeabilization
    No
    Specification
    mesothelioma
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 04 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mesothelin antibody [SP74]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mesentery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica BOND ER2
    Permeabilization
    No
    Specification
    mesentery
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2020

    Question

    I have gone through the site of http://mybiosource.com,(http://www.mybiosource.com/datasheet.php?products_id=427195&pfd=true) ,i have clear so many things by visiting that site, actuallythis mesothelin peptide has a corresponding antibody as i have read on the site, so its somewhat clear in my mind that SP74 clone is a type of clone, it can be raised against the same peptidebcoz the sequence length of this peptide and SP74 clone is exactly similar this product is perhaps useful to us, if u provide the sequence of this clone. as sequence of the MSLN peptide is CPSGKKAREIDES & its corresponding Ab has the same sequence so i want to now clear out this thing. kindly provide the sequence od SP74 clone ASAP. for further action.

    Read More
    Answer


    The peptide from mybiosource is a different peptide immunogen than the one used to produce our product ab93620. As I mentioned the exact immunogen sequence used to produce ab93620 is considered proprietary by our supplying lab however this peptide sequence is found within amino acid region aa520-606 of Q13421 (Uniprot).
    I have compared the peptide sequence from mybiosource to the amino acid sequence of mesothelin and found it to be located between amino acids 300 - 320 of this sequence. This peptide would not bind ab93620.

    Read More

    Question

    in our experiment we are using an antibody (ab93620) dilution 1:100 times in PBS
    buffer as well as same or somewhat different antigen/protein
    concentration again in pbs buffer. then after making dilutions we
    check the specific binding of both the ab-ag interaction/ complex
    through our biosensor unit. it means we need already prepared protein
    for your antibody (ab93620), Abcam company will also provide antigen/
    protein against that antibody (ab93620)? bcoz if it is not so then against which
    source they have raised that particular antibody. i think u will
    understand what i want to explain you.

    Read More
    Answer

    Unfortunately, we do not have any purified mesothelin for sale. I suggest consulting www.biocompare.com. It is a search service that has access to the catalogues of many companies, one of which may have the purified protein you need.

    Read More

    Question

    I have required information about Mesothelin SP74 protein/ antigen,which should be corresponding / Non-corresponding to mesothelin (ab93620 ).

    Read More
    Answer

    The exact immunogen sequence is confidential. However I can say it is located within aa520-606 of Q13421 (Uniprot).

    Read More

    Question

    I have required information for antigen against your abcam antibody Cat# ab93620..This antibody is raised in which source (Antigen OR Protein).

    Read More
    Answer

    Thanks for your reply.
    This antibody is raised against a small synthetic peptide of 15-20 amino acids.
    I hope this is the information you were looking for. Please contact me again if you have any further questions.

    Read More

    Question

    Inquiry: I have required information about Mesothelin SP74 protein/ antigen,which should be corresponding / Non-corresponding to mesothelin (ab93620 ).

    Read More
    Answer

    Thank you for your enquiry but I don't quite understand what you are asking. Are you looking for a blocking peptide?
    Thanks in advance for clarification!

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com