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  1. Link

    msh6-antibody-epr3945-ab92471.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Mismatch Repair
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MSH6 antibody [EPR3945] (ab92471)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
  • OI-RD Scanning - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
  • Anti-MSH6 antibody [EPR3945] (ab92471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3945] to MSH6
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

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Knockout
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Human MSH6 knockout HeLa cell line (ab255410)

View more associated products

Overview

  • Product name

    Anti-MSH6 antibody [EPR3945]
    See all MSH6 primary antibodies
  • Description

    Rabbit monoclonal [EPR3945] to MSH6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MSH6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P52701

  • Positive control

    • WB: A431, HeLa, HAP1 and SW480 cell lysates; Rat brain lysate. IHC-P: Human colon and colonic adenocarcinoma tissue; Rat liver tissue ICC/IF: HeLa and HAP1 cells.
  • General notes

    To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.30 x 10 -9 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3945
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Mismatch Repair

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-MSH6 antibody [EPR3945] (ab198334)
    • Anti-MSH6 antibody [EPR3945] - BSA and Azide free (ab214454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MSH6 knockout HeLa cell line (ab255410)
  • KO cell lysates

    • Human MSH6 knockout HeLa cell lysate (ab263763)
  • KO cell pellets

    • Human MSH6 knockout HeLa cell pellet (ab278881)
  • Recombinant Protein

    • Recombinant Human MSH6 protein (ab152443)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 153 kDa.
IHC-P (1)
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 153 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
  • Involvement in disease

    Defects in MSH6 are the cause of hereditary non-polyposis colorectal cancer type 5 (HNPCC5) [MIM:600678]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Defects in MSH6 are also found in familial colorectal cancers (suspected or incomplete HNPCC) that do not fulfill the Amsterdam criteria for HNPCC.
    Defects in MSH6 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • Sequence similarities

    Belongs to the DNA mismatch repair mutS family.
    Contains 1 PWWP domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P52701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2956 Human
    • Entrez Gene: 17688 Mouse
    • Entrez Gene: 100360342 Rat
    • Omim: 600678 Human
    • SwissProt: P52701 Human
    • SwissProt: P54276 Mouse
    • Unigene: 445052 Human
    • Unigene: 18210 Mouse
    • Alternative names

      • DNA mismatch repair protein Msh6 antibody
      • G/T mismatch binding protein antibody
      • G/T mismatch-binding protein antibody
      • GTBP antibody
      • GTMBP antibody
      • hMSH6 antibody
      • HNPCC 5 antibody
      • HNPCC5 antibody
      • HSAP antibody
      • MSH 6 antibody
      • MSH6 antibody
      • MSH6_HUMAN antibody
      • mutS (E. coli) homolog 6 antibody
      • MutS alpha 160 kDa subunit antibody
      • MutS homolog 6 (E. coli) antibody
      • mutS homolog 6 antibody
      • MutS-alpha 160 kDa subunit antibody
      • p160 antibody
      • Sperm associated protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)

      Immunohistochemical staining of paraffin embedded human colon with purified ab92471 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      All lanes : Anti-MSH6 antibody [EPR3945] (ab92471) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : MSH6 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 153 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab92471 observed at 160 kDa. Red - Anti-Vinculin antibody [VIN-54] observed at 124 kDa.

       ab92471 was shown to react with MSH6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255410 (knockout cell lysate ab263763) was used. Wild-type HeLa and MSH6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92471 and Anti-Vinculin antibody [VIN-54] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      All lanes : Anti-MSH6 antibody [EPR3945] (ab92471) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 cell lysate
      Lane 2 : MSH6 knockout HAP1 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 153 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab92471 observed at 160 kDa. Red - loading control, ab18058, observed at 124 kDa.


      ab92471 was shown to specifically react with MSH6 in wild-type HAP1 cells. No band was observed when MSH6 knockout samples were used. Wild-type and MSH6 knockout samples were subjected to SDS-PAGE. ab92471 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)

      ab92471 staining MSH6 in wild-type HAP1 cells (top panel) and MSH6 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92471 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)

      Immunohistochemical staining of paraffin embedded rat liver with purified ab92471 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)

      ab92471 staining MSH6 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92471 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Anti-MSH6 antibody [EPR3945] (ab92471) at 1/1000 dilution (purified) + Rat brain at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 153 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)This image is courtesy of an Abreview by Serena Bologna.
      All lanes : Anti-MSH6 antibody [EPR3945] (ab92471) at 1/1000 dilution

      All lanes : HAP1 cell line

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : donkey anti-rabbit IgG-HRP at 1/3000 dilution

      Developed using the ECL technique.

      Predicted band size: 153 kDa


      Exposure time: 5 minutes

      See Abreview

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      purified at 1/6000 dilution + SW480 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 153 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Different batches of ab92471 were tested on Rat brain lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 160 kDa.
    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Anti-MSH6 antibody [EPR3945] (ab92471) at 1/2000 dilution (unpurified) + SW480 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 153 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)

      Unpurified ab92471, at a 1/100 dilution, detecting MSH6 in paraffin embedded Human colonic adenocarcinoma tissue by immunohistochemistry. Detection used HRP conjugated anti rabbit antibody.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945] (ab92471)Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

      Unpurified ab92471 (1/500) staining MSH6 in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see abreview.

      See Abreview

    • Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      Western blot - Anti-MSH6 antibody [EPR3945] (ab92471)
      All lanes : Anti-MSH6 antibody [EPR3945] (ab92471) at 1/1000 dilution (unpurified)

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 153 kDa



      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • OI-RD Scanning - Anti-MSH6 antibody [EPR3945] (ab92471)
      OI-RD Scanning - Anti-MSH6 antibody [EPR3945] (ab92471)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)

      Tissue Microarrays stained for "Anti-MSH6 antibody [EPR3945]” using "ab92471" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab92471 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (ab92471)

      Tissue Microarrays stained for "Anti-MSH6 antibody [EPR3945]” using "ab92471" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab92471 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Anti-MSH6 antibody [EPR3945] (ab92471)
      Anti-MSH6 antibody [EPR3945] (ab92471)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (35)

    Publishing research using ab92471? Please let us know so that we can cite the reference in this datasheet.

    ab92471 has been referenced in 35 publications.

    • van Herwaarden YJ  et al. RNF43 mutation analysis in serrated polyposis, sporadic serrated polyps and Lynch syndrome polyps. Histopathology 78:749-758 (2021). PubMed: 33098683
    • Takano S  et al. Clinical significance of genetic alterations in endoscopically obtained pancreatic cancer specimens. Cancer Med 10:1264-1274 (2021). PubMed: 33455072
    • Zhou Z  et al. lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis. Aging (Albany NY) 13:9838-9858 (2021). PubMed: 33744866
    • Hu M  et al. Altered expression of DNA damage repair genes in the brain tissue of mice conceived by in vitro fertilization. Mol Hum Reprod 26:141-153 (2020). PubMed: 32003796
    • Wang JB  et al. An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer. Nat Commun 11:6352 (2020). PubMed: 33311518
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MSH6 antibody [EPR3945]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (endometrial cancer)
    Specification
    endometrial cancer
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 23 2013

    Western blot abreview for Anti-MSH6 antibody [EPR3945]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphoblastoid cell line)
    Loading amount
    20 µg
    Specification
    Lymphoblastoid cell line
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 15 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MSH6 antibody [EPR3945]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Apr 12 2012

    Western blot abreview for Anti-MSH6 antibody [EPR3945]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela wt and MSH6 knockout cells)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% BIS-TRIS gradient, MOPS running buffer)
    Loading amount
    20 µg
    Specification
    Hela wt and MSH6 knockout cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 22 2021

    Western blot abreview for Anti-MSH6 antibody [EPR3945]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Serena Bologna

    Verified customer

    Submitted Oct 10 2016

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