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    muc2-antibody-epr23479-47-ab272692.pdf

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RecombinantRabMAb

Recombinant Anti-MUC2 antibody [EPR23479-47] (ab272692)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
  • Anti-MUC2 antibody [EPR23479-47] (ab272692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23479-47] to MUC2
  • Suitable for: IHC-P, mIHC, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-MUC2 antibody [EPR23479-47]
    See all MUC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR23479-47] to MUC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, mIHC, WB, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon cancer tissue lysate. Mouse small intestine tissue lysate. Rat small intestine and colon tissue lysate. IHC-P: Human, mouse and rat colon tissue. Human bladder cancer tissue. IHC-Fr: Mouse and rat colon tissue. mIHC: Human colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23479-47
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Associated products

  • Alternative Versions

    • Anti-MUC2 antibody [EPR23479-47] - BSA and Azide free (ab272706)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Related Products

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
mIHC
Use at an assay dependent concentration.
WB (1)
1/1000. Predicted molecular weight: 540 kDa.
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
mIHC
Use at an assay dependent concentration.
WB
1/1000. Predicted molecular weight: 540 kDa.
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications

    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Target information above from: UniProt accession Q02817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4583 Human
    • Entrez Gene: 17831 Mouse
    • Entrez Gene: 24572 Rat
    • Omim: 158370 Human
    • SwissProt: Q02817 Human
    • SwissProt: Q80Z19 Mouse
    • SwissProt: Q62635 Rat
    • Unigene: 315 Human
    • Unigene: 2041 Mouse
    • Unigene: 461296 Mouse
    • Unigene: 195421 Rat
    see all
  • Alternative names

    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Villin (ab245749, gray; Opal™690), anti-liver FABP (ab240401, green; Opal™520) and anti-MUC2 (ab272692, red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Villin stained on apical border. Panel D: anti-MUC2 stained on goblet cells. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab245749 (1/1000 dilution), ab240401 (1/8000 dilution), and ab272692 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    All lanes : Anti-MUC2 antibody [EPR23479-47] (ab272692) at 1/1000 dilution

    Lane 1 : Human colon cancer tissue lysate
    Lane 2 : Mouse small intestine tissue lysate
    Lane 3 : Rat small intestine tissue lysate
    Lane 4 : Rat colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 540 kDa
    Observed band size: 140-170 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    MUC2 is highly glycosylated.

    The C-terminal fragment alpha-MUC2C2 (140-170 kDa) is observed. The molecular weight is consistent with what have been described in literature (PMID: 12582180; 16754877).  Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on rat colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human bladder cancer (PMID: 25197366). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human colon (PMID: 28693267). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Anti-MUC2 antibody [EPR23479-47] (ab272692)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (4)

Publishing research using ab272692? Please let us know so that we can cite the reference in this datasheet.

ab272692 has been referenced in 4 publications.

  • Chen K  et al. Distinct contributions of cathelin-related antimicrobial peptide (CRAMP) derived from epithelial cells and macrophages to colon mucosal homeostasis. J Pathol 253:339-350 (2021). PubMed: 33104252
  • Deng M  et al. miR-452-5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl-1. Exp Ther Med 22:813 (2021). PubMed: 34131436
  • Liu Y  et al. Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19. Cell Res 30:1078-1087 (2020). PubMed: 33159154
  • Hou Q  et al. Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b. Commun Biol 3:611 (2020). PubMed: 33097830

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-MUC2 antibody [EPR23479-47]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (colon)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
15 µg
Specification
colon
Blocking step
Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 26 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC2 antibody [EPR23479-47]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Gut)
Antigen retrieval step
None
Permeabilization
No
Specification
Gut
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Fixative
Methacarn-PE
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 25 2021

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