Recombinant Anti-MUC2 antibody [EPR23479-47] (ab272692)
![Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-494150-antiliver-fabp-antibody-bsa-and-azide-free-epr20464-multiplex-immunohistochemistry-2-human-ab240401.jpg "Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23479-47] to MUC2
- Suitable for: IHC-P, mIHC, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MUC2 antibody [EPR23479-47]
See all MUC2 primary antibodies -
Description
Rabbit monoclonal [EPR23479-47] to MUC2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, mIHC, WB, IHC-Frmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human colon cancer tissue lysate. Mouse small intestine tissue lysate. Rat small intestine and colon tissue lysate. IHC-P: Human, mouse and rat colon tissue. Human bladder cancer tissue. IHC-Fr: Mouse and rat colon tissue. mIHC: Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23479-47 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| mIHC |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000. Predicted molecular weight: 540 kDa.
|
| IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
mIHC
Use at an assay dependent concentration. |
|
WB
1/1000. Predicted molecular weight: 540 kDa. |
|
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Target
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Function
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
Tissue specificity
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
Post-translational
modificationsO-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
Cellular localization
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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Database links
- Entrez Gene: 4583 Human
- Entrez Gene: 17831 Mouse
- Entrez Gene: 24572 Rat
- Omim: 158370 Human
- SwissProt: Q02817 Human
- SwissProt: Q80Z19 Mouse
- SwissProt: Q62635 Rat
- Unigene: 315 Human
see all -
Alternative names
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
Images
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Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Villin (ab245749, gray; Opal™690), anti-liver FABP (ab240401, green; Opal™520) and anti-MUC2 (ab272692, red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Villin stained on apical border. Panel D: anti-MUC2 stained on goblet cells. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab245749 (1/1000 dilution), ab240401 (1/8000 dilution), and ab272692 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
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![Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-6-anti-muc2-antibody-epr2347947-immunohistochemistry-frozen-rat-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-5-anti-muc2-antibody-epr2347947-immunohistochemistry-frozen-mouse-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-390216-anti-muc2-antibody-epr2347947-western-blot-human-colon-cancer-tissue-lysate20-ug.jpg)
Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)All lanes : Anti-MUC2 antibody [EPR23479-47] (ab272692) at 1/1000 dilution
Lane 1 : Human colon cancer tissue lysate
Lane 2 : Mouse small intestine tissue lysate
Lane 3 : Rat small intestine tissue lysate
Lane 4 : Rat colon tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 540 kDa
Observed band size: 140-170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MUC2 is highly glycosylated.
The C-terminal fragment alpha-MUC2C2 (140-170 kDa) is observed. The molecular weight is consistent with what have been described in literature (PMID: 12582180; 16754877). Exposure time: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-4-anti-muc2-antibody-epr2347947-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on rat colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-3-anti-muc2-antibody-epr2347947-immunohistochemistry-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-2-anti-muc2-antibody-epr2347947-immunohistochemistry-human-bladder-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human bladder cancer (PMID: 25197366). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-1-anti-muc2-antibody-epr2347947-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human colon (PMID: 28693267). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-MUC2 antibody [EPR23479-47] (ab272692)](https://www.abcam.com/ps/products/272/ab272692/Images/ab272692-8-benefits-of-recombinant-antibodies.png)
Anti-MUC2 antibody [EPR23479-47] (ab272692)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab272692 has been referenced in 4 publications.
- Chen K et al. Distinct contributions of cathelin-related antimicrobial peptide (CRAMP) derived from epithelial cells and macrophages to colon mucosal homeostasis. J Pathol 253:339-350 (2021). PubMed: 33104252
- Deng M et al. miR-452-5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl-1. Exp Ther Med 22:813 (2021). PubMed: 34131436
- Liu Y et al. Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19. Cell Res 30:1078-1087 (2020). PubMed: 33159154
- Hou Q et al. Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b. Commun Biol 3:611 (2020). PubMed: 33097830