Recombinant Anti-NDRG1 antibody [EPR5593] KO Tested (ab124689)

Product name

Anti-NDRG1 antibody [EPR5593]
See all NDRG1 primary antibodies
Description

Rabbit monoclonal [EPR5593] to NDRG1
Host species

Rabbit
Specificity

PBS only lot tested.
Tested applications

Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human NDRG1 aa 350-450 (C terminal). The exact sequence is proprietary.
Positive control
- IHC-P: Human colon tissue, cervical carcinoma tissue, colonic adenocarcinoma tissue, normal kidney tissue and breast carcinoma tissue. ICC/IF: HeLa. IP: HeLa. WB: Wild-type HEK-293 whole cell lysate. Jurkat, HeLa, Caco-2 and LnCap whole cell lysate. Mouse and rat brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.33 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5593
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human NDRG1 knockout HEK-293 cell line (ab261856)
- Human NDRG1 knockout HEK-293T cell line (ab267301)
KO cell lysates
- Human NDRG1 knockout HEK-293T cell lysate (ab257551)
- Human NDRG1 knockout HEK-293 cell lysate (ab261664)
KO cell pellets
- Human NDRG1 knockout HEK-293T cell pellet (ab278943)
Positive Controls
- HeLa nuclear extract lysate (ab14655)
- HeLa membrane extract lysate (ab29547)
Recombinant Protein
- Recombinant human NDRG1 protein (ab167974)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IP		1/10 - 1/100.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

Notes
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt.

Function

May have a growth inhibitory role.
Tissue specificity

Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Involvement in disease

Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
Sequence similarities

Belongs to the NDRG family.
Cellular localization

Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
Target information above from: UniProt accession Q92597 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10397 Human
- Entrez Gene: 17988 Mouse
- Entrez Gene: 299923 Rat
- Omim: 605262 Human
- SwissProt: Q92597 Human
- SwissProt: Q62433 Mouse
- SwissProt: Q6JE36 Rat
- Unigene: 372914 Human
- Unigene: 30837 Mouse
- Unigene: 153992 Rat
see all
Alternative names
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
- cmt4d antibody
- Differentiation related gene1 protein antibody
- Differentiation-related gene 1 protein antibody
- Drg 1 antibody
- DRG-1 antibody
- drg1 antibody
- gc4 antibody
- GC4 antibody antibody
- hmsnl antibody
- Human mRNA for RTP complete cds antibody
- N myc downstream regulated gene 1 antibody
- N myc downstream regulated gene 1 protein antibody
- N-myc downstream-regulated gene 1 protein antibody
- Ndr 1 antibody
- ndr1 antibody
- NDRG 1 antibody
- Ndrg1 antibody
- NDRG1 protein antibody
- NDRG1_HUMAN antibody
- Nickel specific induction protein antibody
- Nickel specific induction protein Cap43 antibody
- Nickel-specific induction protein Cap43 antibody
- nmsl antibody
- Nmyc downstream regulated antibody
- Nmyc downstream regulated gene1 antibody
- Nmyc downstream regulated gene1 protein antibody
- Protein NDRG1 antibody
- Protein regulated by oxygen 1 antibody
- Protein regulated by oxygen1 antibody
- Proxy1 antibody
- Reduced in tumor antibody
- Reducin antibody
- Reducing agents and tunicamycin responsive protein antibody
- Reducing agents and tunicamycin-responsive protein antibody
- Rit42 antibody
- RTP antibody
- targ1 antibody
- TDD5 antibody
- tdds antibody
- Tunicamycin responsive protein antibody
see all

Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)

All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : NDRG1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1-4: Merged signal (red and green). Green - ab124689 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab124689 Anti-NDRG1 antibody [EPR5593] was shown to specifically react with NDRG1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267301 (knockout cell lysate ab257551) was used. Wild-type and NDRG1 knockout samples were subjected to SDS-PAGE. ab124689 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689 at 1/100 dilution, staining NDRG1 in Formalin fixed Paraffin-embedded human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)

All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution

Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : NDRG1 knockout HEK-293 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LnCap whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 43 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab124689 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab124689 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in NDRG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NDRG1 knockout samples were subjected to SDS-PAGE. Ab124689 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)

All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 0.0083 µg/ml

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Exposure time: 3 minutes
Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] (ab124689)

Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.

Lane 2 (+): ab124689 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124689 in HeLa whole cell lysate

ab124689 (Purified) at 1/50 dilution (20µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, ab124689 at 1/500 dilution (1.86 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Blocking and diluting buffer: 5% NFDM /TBST .
OI-RD Scanning - Anti-NDRG1 antibody [EPR5593] (ab124689)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)

All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution

Lane 1 : CaCo-2 (Human colorectal adenocarcinoma cell line) lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : LnCaP (Human prostate cancer cell line) lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689, at 1/100 dilution, staining NDRG1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689 showing positive staining in human cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689 showing positive staining in human colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689 showing positive staining in human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)

ab124689 showing positive staining in human breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-NDRG1 antibody [EPR5593] (ab124689)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab124689? Please let us know so that we can cite the reference in this datasheet.

ab124689 has been referenced in 13 publications.

Watari K et al. NDRG1 activates VEGF-A-induced angiogenesis through PLC?1/ERK signaling in mouse vascular endothelial cells. Commun Biol 3:107 (2020). PubMed: 32144393
Shen L et al. Tac2-N serves an oncogenic role and promotes drug resistance in human gastric cancer cells. Exp Ther Med 20:113 (2020). PubMed: 32989391
Dong X et al. NDRG1 suppresses vasculogenic mimicry and tumor aggressiveness in gastric carcinoma. Oncol Lett 18:3003-3016 (2019). PubMed: 31452779
Zhang S et al. N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21. J Exp Clin Cancer Res 38:490 (2019). PubMed: 31831018
Zhang F et al. NDRG1 facilitates the replication and persistence of Kaposi's sarcoma-associated herpesvirus by interacting with the DNA polymerase clamp PCNA. PLoS Pathog 15:e1007628 (2019). PubMed: 30811506

View all Publications for this product

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

KO cell pellets

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (13)

Customer reviews and Q&As

Dissociation constant (K_D)