Recombinant Anti-NDRG1 antibody [EPR5593] (ab124689)
![Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-412775-anti-ndrg1-antibody-epr5593-western-blot-human-knockout-lysate.jpg "Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5593] to NDRG1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NDRG1 antibody [EPR5593]
See all NDRG1 primary antibodies -
Description
Rabbit monoclonal [EPR5593] to NDRG1 -
Host species
Rabbit -
Specificity
PBS only lot tested. -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NDRG1 aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- IHC-P: Human colon tissue, cervical carcinoma tissue, colonic adenocarcinoma tissue, normal kidney tissue and breast carcinoma tissue. ICC/IF: HeLa. IP: HeLa. WB: Wild-type HEK-293 whole cell lysate. Jurkat, HeLa, Caco-2 and LnCap whole cell lysate. Mouse and rat brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.33 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5593 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
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| IP |
1/10 - 1/100.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa). |
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IP
1/10 - 1/100. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
May have a growth inhibitory role. -
Tissue specificity
Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression. -
Involvement in disease
Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. -
Sequence similarities
Belongs to the NDRG family. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro. - Information by UniProt
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Database links
- Entrez Gene: 10397 Human
- Entrez Gene: 17988 Mouse
- Entrez Gene: 299923 Rat
- Omim: 605262 Human
- SwissProt: Q92597 Human
- SwissProt: Q62433 Mouse
- SwissProt: Q6JE36 Rat
- Unigene: 372914 Human
see all -
Alternative names
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
see all
Images
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Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : NDRG1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1-4: Merged signal (red and green). Green - ab124689 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124689 Anti-NDRG1 antibody [EPR5593] was shown to specifically react with NDRG1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267301 (knockout cell lysate ab257551) was used. Wild-type and NDRG1 knockout samples were subjected to SDS-PAGE. ab124689 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138457-anti-ndrg1-antibody-epr5593-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689 at 1/100 dilution, staining NDRG1 in Formalin fixed Paraffin-embedded human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-365511-anti-ndrg1-antibody-epr5593-western-blot-knockout-hek-293-ndrg1-jurkat-lncap-human-lysates.jpg)
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : NDRG1 knockout HEK-293 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LnCap whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDaLanes 1 - 4: Merged signal (red and green). Green - ab124689 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab124689 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in NDRG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NDRG1 knockout samples were subjected to SDS-PAGE. Ab124689 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-308636-anti-ndrg1-antibody-epr5593-western-blot.jpg)
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 0.0083 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Exposure time: 3 minutes -
![Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-309039-anti-ndrg1-antibody-epr5593-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] (ab124689)Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.
Lane 2 (+): ab124689 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124689 in HeLa whole cell lysate
ab124689 (Purified) at 1/50 dilution (20µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, ab124689 at 1/500 dilution (1.86 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
![OI-RD Scanning - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138451-anti-ndrg1-antibody-epr5593.jpg)
OI-RD Scanning - Anti-NDRG1 antibody [EPR5593] (ab124689) -
![Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138458-anti-ndrg1-antibody-epr5593-western-blot.jpg)
Western blot - Anti-NDRG1 antibody [EPR5593] (ab124689)All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution
Lane 1 : CaCo-2 (Human colorectal adenocarcinoma cell line) lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : LnCaP (Human prostate cancer cell line) lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138456-anti-ndrg1-antibody-epr5593-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689, at 1/100 dilution, staining NDRG1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138455-anti-ndrg1-antibody-epr5593-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689 showing positive staining in human cervical carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138454-anti-ndrg1-antibody-epr5593-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689 showing positive staining in human colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138453-anti-ndrg1-antibody-epr5593-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689 showing positive staining in human normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-138452-anti-ndrg1-antibody-epr5593-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (ab124689)ab124689 showing positive staining in human breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NDRG1 antibody [EPR5593] (ab124689)](https://www.abcam.com/ps/products/124/ab124689/Images/ab124689-13-benefits-of-recombinant-antibodies.png)
Anti-NDRG1 antibody [EPR5593] (ab124689)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab124689 has been referenced in 15 publications.
- Dong L et al. NDRG1 facilitates lytic replication of Kaposi's sarcoma-associated herpesvirus by maintaining the stability of the KSHV helicase. PLoS Pathog 17:e1009645 (2021). PubMed: 34077484
- Zeng F et al. Employing hypoxia characterization to predict tumour immune microenvironment, treatment sensitivity and prognosis in hepatocellular carcinoma. Comput Struct Biotechnol J 19:2775-2789 (2021). PubMed: 34093992
- Watari K et al. NDRG1 activates VEGF-A-induced angiogenesis through PLC?1/ERK signaling in mouse vascular endothelial cells. Commun Biol 3:107 (2020). PubMed: 32144393
- Shen L et al. Tac2-N serves an oncogenic role and promotes drug resistance in human gastric cancer cells. Exp Ther Med 20:113 (2020). PubMed: 32989391
- Dong X et al. NDRG1 suppresses vasculogenic mimicry and tumor aggressiveness in gastric carcinoma. Oncol Lett 18:3003-3016 (2019). PubMed: 31452779