Recombinant Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)
![Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-230001-anti-nesprin1-syne-1-antibody-epr14196-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nesprin1/Syne-1 antibody [EPR14196]
See all Nesprin1/Syne-1 primary antibodies -
Description
Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293, HACAT, RAW 264.7, PC12 and NIH 3T3 cell lysates; human kidney, human bladder transitional cell carcinoma and rat stomach tissues; A673 and HACAT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14196 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/50.
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| WB |
1/1000 - 1/20000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/50. |
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WB
1/1000 - 1/20000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Required for centrosome migration to the apical cell surface during early ciliogenesis. -
Tissue specificity
Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, and peripheral blood leukocytes. -
Involvement in disease
Defects in SYNE1 are the cause of spinocerebellar ataxia autosomal recessive type 8 (SCAR8) [MIM:610743]; also known as autosomal recessive cerebellar ataxia type 1 (ARCA1) or recessive ataxia of Beauce. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR8 is an autosomal recessive form.
Defects in SYNE1 are the cause of Emery-Dreifuss muscular dystrophy type 4 (EDMD4) [MIM:612998]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows, Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. -
Sequence similarities
Belongs to the nesprin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 12 HAT repeats.
Contains 1 KASH domain.
Contains 31 spectrin repeats. -
Domain
The KASH domain, which contains a transmembrane domain, mediates the nuclear envelope targeting and is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. -
Cellular localization
Nucleus outer membrane. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. In skeletal and smooth muscles, a significant amount is found in the sarcomeres. - Information by UniProt
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Database links
- Entrez Gene: 23345 Human
- Entrez Gene: 64009 Mouse
- Entrez Gene: 499010 Rat
- Omim: 608441 Human
- SwissProt: Q8NF91 Human
- SwissProt: Q6ZWR6 Mouse
- Unigene: 12967 Human
- Unigene: 331626 Mouse
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Alternative names
- 8B antibody
- ARCA1 antibody
- C6orf98 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)
Immunofluorescent analysis of A673 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Nesprin1/Syne-1 with ab192234 at 1/50 dilution (4.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution; counter-stained with DAPI (blue).
Negative controls: anti-Nesprin1/Syne-1 at 1/50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Flow Cytometry (Intracellular) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-230002-anti-nesprin1-syne-1-antibody-epr14196-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)Intracellular flow cytometric analysis of HACAT cells (paraformaldehyde-fixed, 2%) labeling Nesprin1/Syne-1 with ab192234 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-230000-anti-nesprin1-syne-1-antibody-epr14196-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-229999-anti-nesprin1-syne-1-antibody-epr14196-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-229998-anti-nesprin1-syne-1-antibody-epr14196-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-229997-anti-nesprin1-syne-1-antibody-epr14196-western-blot.jpg)
Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)All lanes : Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/1000 dilution
Lane 1 : RAW 264.7 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 112 kDa -
![Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-229996-anti-nesprin1-syne-1-antibody-epr14196-western-blot.jpg)
Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)All lanes : Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 112 kDa -
![Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)](https://www.abcam.com/ps/products/192/ab192234/Images/ab192234-8-benefits-of-recombinant-antibodies.png)
Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab192234 has been referenced in 4 publications.
- Ross JA et al. Impairments in contractility and cytoskeletal organisation cause nuclear defects in nemaline myopathy. Acta Neuropathol 138:477-495 (2019). PubMed: 31218456
- Wada E et al. Deficiency of emerin contributes differently to the pathogenesis of skeletal and cardiac muscles in LmnaH222P/H222P mutant mice. PLoS One 14:e0221512 (2019). PubMed: 31430335
- Brody MJ et al. Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis. J Biol Chem 294:8918-8929 (2019). PubMed: 31006653
- Levy Y et al. Prelamin A causes aberrant myonuclear arrangement and results in muscle fiber weakness. JCI Insight 3:N/A (2018). PubMed: 30282816