Recombinant Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)
![Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-445232-AntinNOS-neuronal-antibody-ICC.jpg "Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1855Y] to nNOS (neuronal)
- Suitable for: ICC, Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-nNOS (neuronal) antibody [EP1855Y]
See all nNOS (neuronal) primary antibodies -
Description
Rabbit monoclonal [EP1855Y] to nNOS (neuronal) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cyt (Intra), WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human, Common marmoset
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain tissue lysate. ICC: PC-12 cells. Flow Cyt (intra): PC-12 cells. IP: Rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1855Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/200.
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| Flow Cyt (Intra) |
1/600.
For unpurified use at 1/15. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (6) |
1/1000 - 1/2000. Predicted molecular weight: 161 kDa.
For unpurified use at 1/100. |
| IP |
1/150.
For unpurified use at 1/4. |
| Notes |
|---|
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ICC
1/200. |
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Flow Cyt (Intra)
1/600. For unpurified use at 1/15. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/2000. Predicted molecular weight: 161 kDa. For unpurified use at 1/100. |
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IP
1/150. For unpurified use at 1/4. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
Post-translational
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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Database links
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: P29476 Rat
- Unigene: 654410 Human
see all -
Alternative names
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
Images
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Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)
Immunocytochemistry analysis of PC-12 cells labelling nNOS (neuronal) (green) with purified ab76067 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
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![Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240065-ab76067upwb.jpg)
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/100 dilution (unpurified) + Mouse brain tissue lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 161 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240066-ab76067pwb.jpg)
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/3000 dilution (purified) + Mouse brain tissue lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 161 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067_1.JPG)
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/1000 dilution (unpurified) + Mouse brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 161 kDa
Observed band size: 161 kDa -
![Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/nNOS-neuronal-Primary-antibodies-ab76067-1.jpg)
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Overlay histogram showing PC-12 cells stained with unpurified ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76067, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC-12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
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![Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240068-ab76067upfc.jpg)
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with unpurified ab76067 at 1/15 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
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![Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240069-ab76067pfc.jpg)
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with purified ab76067 at 1/600 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
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![Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240070-ab76067upip.jpg)
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)ab76067 (unpurified) at 1/4 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-240071-ab76067pip.jpg)
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)ab76067 (purified) at 1/150 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)](https://www.abcam.com/ps/products/76/ab76067/Images/ab76067-13-benefits-of-recombinant-antibodies.png)
Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (53)
ab76067 has been referenced in 53 publications.
- Tan M et al. Maternal vitamin A deficiency impairs cholinergic and nitrergic neurons, leading to gastrointestinal dysfunction in rat offspring via RARß. Life Sci 264:118688 (2021). PubMed: 33130074
- Mosqueira M et al. Cardiomyocyte depolarization triggers NOS-dependent NO transient after calcium release, reducing the subsequent calcium transient. Basic Res Cardiol 116:18 (2021). PubMed: 33728868
- Yuan H et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
- Mikhailik A et al. nNOS regulates ciliated cell polarity, ciliary beat frequency, and directional flow in mouse trachea. Life Sci Alliance 4:N/A (2021). PubMed: 33653689
- Liang L et al. Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther 12:87 (2021). PubMed: 33494812