Name: Anti-nNOS (neuronal) antibody [EP1855Y]
Brand: RabMAb
SKU: ab76067
Rating: 5 (10 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1855Y] to nNOS (neuronal)
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
Reacts with: Mouse, Rat

Alexa Fluor® 594 Carrier Free

Product name

Anti-nNOS (neuronal) antibody [EP1855Y]
See all nNOS (neuronal) primary antibodies
Description

Rabbit monoclonal [EP1855Y] to nNOS (neuronal)
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat
Predicted to work with: Human, Common marmoset
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain tissue lysate. ICC/IF: PC-12 cells. Flow Cyt (intra): PC-12 cells. IP: Rat brain tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1855Y
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/600. For unpurified use at 1/15. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(6)	1/1000 - 1/2000. Predicted molecular weight: 161 kDa. For unpurified use at 1/100.
IP		1/150. For unpurified use at 1/4.
ICC/IF	(1)	1/200.

Notes
Flow Cyt (Intra) 1/600. For unpurified use at 1/15. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/2000. Predicted molecular weight: 161 kDa. For unpurified use at 1/100.
IP 1/150. For unpurified use at 1/4.
ICC/IF 1/200.

Application notes

Is unsuitable for IHC-P.

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Tissue specificity

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Sequence similarities

Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.
Domain

The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
Post-translational
modifications

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cellular localization

Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Target information above from: UniProt accession P29475 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: P29476 Rat
- Unigene: 654410 Human
- Unigene: 684465 Human
- Unigene: 684466 Human
- Unigene: 684467 Human
- Unigene: 442195 Mouse
- Unigene: 44249 Mouse
- Unigene: 10573 Rat
see all
Alternative names
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
- EC 1.14.13.39 antibody
- IHPS 1 antibody
- IHPS1 antibody
- N-NOS antibody
- NC-NOS antibody
- neuronal Nitric Oxide Synthase antibody
- Neuronal NOS antibody
- Nitric oxide synthase , neuronal, included antibody
- Nitric oxide synthase 1 (neuronal) antibody
- Nitric oxide synthase 1 antibody
- Nitric oxide synthase, brain antibody
- Nitric oxide synthase, penile neuronal, included antibody
- NNOS antibody
- NO antibody
- NOS 1 antibody
- NOS antibody
- NOS type I antibody
- NOS-I antibody
- NOS1 antibody
- NOS1_HUMAN antibody
- Peptidyl-cysteine S-nitrosylase NOS1 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Immunocytochemistry analysis of PC-12 cells labelling nNOS (neuronal) (green) with purified ab76067 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/100 dilution (unpurified) + Mouse brain tissue lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 161 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/3000 dilution (purified) + Mouse brain tissue lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 161 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/1000 dilution (unpurified) + Mouse brain tissue lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 161 kDa
Observed band size: 161 kDa
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Overlay histogram showing PC-12 cells stained with unpurified ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76067, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC-12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with unpurified ab76067 at 1/15 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with purified ab76067 at 1/600 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

ab76067 (unpurified) at 1/4 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

ab76067 (purified) at 1/150 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab76067? Please let us know so that we can cite the reference in this datasheet.

ab76067 has been referenced in 53 publications.

Tan M et al. Maternal vitamin A deficiency impairs cholinergic and nitrergic neurons, leading to gastrointestinal dysfunction in rat offspring via RARß. Life Sci 264:118688 (2021). PubMed: 33130074
Mosqueira M et al. Cardiomyocyte depolarization triggers NOS-dependent NO transient after calcium release, reducing the subsequent calcium transient. Basic Res Cardiol 116:18 (2021). PubMed: 33728868
Yuan H et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
Mikhailik A et al. nNOS regulates ciliated cell polarity, ciliary beat frequency, and directional flow in mouse trachea. Life Sci Alliance 4:N/A (2021). PubMed: 33653689
Liang L et al. Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther 12:87 (2021). PubMed: 33494812

View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-10 of 15 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Rat Tissue (Jejunum myenteric plexus)

Specification

Jejunum myenteric plexus

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 25 2019

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (Brain)

Specification

Brain

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hyun Jin Kim

Verified customer

Submitted Mar 21 2016

Application

Western blot

Sample

Rat Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2016

Application

Western blot

Sample

Rat Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 02 2015

Application

Immunohistochemistry (Frozen sections)

Sample

Common marmoset Tissue sections (Caudate)

Permeabilization

Yes - Triton-X

Specification

Caudate

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 21 2014

Application

Western blot

Sample

Human Tissue lysate - whole (vastus lateralis muscle)

Gel Running Conditions

Reduced Denaturing (8% Gel)

Loading amount

50 µg

Specification

vastus lateralis muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 18 2014

Application

Western blot

Sample

Mouse Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (8)

Loading amount

50 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 09 2014

Application

Western blot

Sample

Human Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (8% Gel)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. G.K Sakellariou

Verified customer

Submitted May 14 2013

Question

Could you provide suitable antibody for neuronal NOS?

Abcam community

Verified customer

Asked on Jul 25 2013

Answer

The NOS1 protein of Mustela is 95% similar to Bovine nNOS so it seems like the antibody ab2801 could be an option. It suppose to detect brain Nitric Oxide Synthase.

As Ferret is an untested species so in case you are interested in buying and trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Padamjeet Singh

Abcam Scientific Support

Answered on Jul 25 2013

Question

die Nachricht über die Verschickungder kostenlosen Ersatzlieferungvon ab76067 für den ersten nNOS-AK ab63602bekam ich am 05.07.2012. Als Referenznummer wurde die 123456 angegeben. Ich hoffe, das hilft Ihnen weiter... Herzlichen Dank schon mal für die Erstattung des Einkaufspreises.

Mit freundlichen Grüßen und ein schönes Wochenende,

Abcam community

Verified customer

Asked on Sep 03 2012

Answer

Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456.

Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutschrift für Sie auszustellen. Sie können diese Gutschrift auf folgende Weise verwenden:

(1) Gegen die Rechnung dieses Antikörpers verwenden falls diese noch nicht bezahlt wurde
(2) Aufbewahren und gegen eine zukünftige Rechnung verwenden
(3) Wir stellen Ihnen gerne eine komplette Rückerstattung aus, wenn keine offenen Rechnungen mehr ausstehen

Um eine Rückerstattung des Kaufpreises zu erhalten oder um zu bestätigen wie Sie diese Gutschrift benutzen möchten, möchte ich Sie bitten, sich mit unserer Finanzabteilung in Verbindung zu setzen.
Sie können unsere Finanzabteilung per Email unter der Adresse creditcontrol@abcam.com oder telefonisch unter der Nummer 019 288 259 erreichen.

Bitte geben Sie die Referenznummer Ihrer Gutschrift immer mit an.

Die Referenznummer ist für Ihre Unterlagen bestimmt. Wir schicken Ihnen die eigentliche Gutschrift per Post oder Email zu. Sie enthält auch die Gutschriftnummer, die mit dem Buchstaben CGB beginnt.

Ich hoffe dass Sie trotz dieser schlechten Erfahrung weiterhin Kunde bei uns bleiben. Bitte zögern Sie nicht sich wieder an uns zu wenden, falls Sie weitere Fragen haben.

Abcam Scientific Support

Answered on Sep 03 2012

1-10 of 15 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (53)

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Immunohistochemistry free floating abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Western blot abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Western blot abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Immunohistochemistry (Frozen sections) abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Western blot abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Western blot abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Western blot abreview for Anti-nNOS (neuronal) antibody [EP1855Y]

Post-translational
modifications