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    nup98-antibody-epr22818-128-ab234434.pdf

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Signal Transduction Protein Trafficking Nuclear Import / Export
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RecombinantRabMAb

Recombinant Anti-NUP98 antibody [EPR22818-128] (ab234434)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
  • Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
  • Anti-NUP98 antibody [EPR22818-128] (ab234434)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22818-128] to NUP98
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human NUP98 protein (ab159019)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-NUP88 antibody (ab79785)

View more associated products

Overview

  • Product name

    Anti-NUP98 antibody [EPR22818-128]
    See all NUP98 primary antibodies
  • Description

    Rabbit monoclonal [EPR22818-128] to NUP98
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, MCF7, HepG2, NIH/3T3, A20, PC-12, RAW264.7 whole cell lysate. Mouse testis and kidney tissue lysate. Human testis tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22818-128
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Alternative Versions

    • Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Recombinant Protein

    • Recombinant Human NUP98 protein (ab159019)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab234434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 198 kDa).
Notes
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 198 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
  • Involvement in disease

    Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
    Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
    Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
    Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
    Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the nucleoporin GLFG family.
    Contains 1 peptidase S59 domain.
  • Domain

    Contains G-L-F-G repeats.
  • Post-translational
    modifications

    Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
  • Target information above from: UniProt accession P52948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4928 Human
    • Entrez Gene: 269966 Mouse
    • Entrez Gene: 81738 Rat
    • Omim: 601021 Human
    • SwissProt: P52948 Human
    • SwissProt: Q6PFD9 Mouse
    • SwissProt: P49793 Rat
    • Unigene: 524750 Human
    • Unigene: 11324 Rat
    see all
  • Alternative names

    • 96 kDa nucleoporin antibody
    • 98 kDa nucleoporin antibody
    • ADAR2 antibody
    • ADIR2 antibody
    • GLFG-repeat containing nucleoporin antibody
    • Nuclear pore complex protein Nup96 antibody
    • Nuclear pore complex protein Nup98 Nup96 antibody
    • Nucleoporin 98kD antibody
    • nucleoporin 98kDa antibody
    • Nucleoporin Nup96 antibody
    • Nucleoporin Nup98 antibody
    • NUP196 antibody
    • NUP96 antibody
    • Nup98 antibody
    • Nup98-Nup96 antibody
    • NUP98_HUMAN antibody
    see all

Images

  • Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
    Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
    All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 198 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?



    Exposure time: Lanes 1-2: 15 seconds Lane 3: 5.5 seconds.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
    Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)
    All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 6 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 8 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 198 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Recommend using fresh lysates to avoid target protein being degraded.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Anti-NUP98 antibody [EPR22818-128] (ab234434)
    Anti-NUP98 antibody [EPR22818-128] (ab234434)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab234434? Please let us know so that we can cite the reference in this datasheet.

ab234434 has not yet been referenced specifically in any publications.

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