Recombinant Anti-NUP98 antibody [EPR22818-128] (ab234434)
![Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)](https://www.abcam.com/ps/products/234/ab234434/Images/ab234434-353127-anti-nup98-antibody-epr-22818-128-western-blot-human-mouse.jpg "Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22818-128] to NUP98
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NUP98 antibody [EPR22818-128]
See all NUP98 primary antibodies -
Description
Rabbit monoclonal [EPR22818-128] to NUP98 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, MCF7, HepG2, NIH/3T3, A20, PC-12, RAW264.7 whole cell lysate. Mouse testis and kidney tissue lysate. Human testis tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22818-128 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 198 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 198 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
Involvement in disease
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
Domain
Contains G-L-F-G repeats. -
Post-translational
modificationsIsoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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Database links
- Entrez Gene: 4928 Human
- Entrez Gene: 269966 Mouse
- Entrez Gene: 81738 Rat
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: Q6PFD9 Mouse
- SwissProt: P49793 Rat
- Unigene: 524750 Human
see all -
Alternative names
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
Images
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Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 198 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 15 seconds Lane 3: 5.5 seconds.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)](https://www.abcam.com/ps/products/234/ab234434/Images/ab234434-353126-anti-nup98-antibody-epr-22818-128-western-blot-human-mouse-rat.jpg)
Western blot - Anti-NUP98 antibody [EPR22818-128] (ab234434)All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 6 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 8 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 198 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsRecommend using fresh lysates to avoid target protein being degraded.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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![Anti-NUP98 antibody [EPR22818-128] (ab234434)](https://www.abcam.com/ps/products/234/ab234434/Images/ab234434-3-benefits-of-recombinant-antibodies.png)
Anti-NUP98 antibody [EPR22818-128] (ab234434)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab234434 has not yet been referenced specifically in any publications.