Recombinant Anti-PDK4 antibody [EPR19727-245] (ab214938)
![Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-266941-anti-pdk4-antibody-epr19727-245-western-blot.jpg "Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19727-245] to PDK4
- Suitable for: IP, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PDK4 antibody [EPR19727-245]
See all PDK4 primary antibodies -
Description
Rabbit monoclonal [EPR19727-245] to PDK4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse PDK4 recombinant protein; mouse skeletal muscle and heart lysates; rat muscle and heart lysates; C2C12 and C6 whole cell lysate; heart from nonfasting mouse and mouse fasting for 72 hours; gastrocnemius muscle from nonfasting mouse and mouse fasting for 72 hours. Flow Cyt (intra): C2C12 cells. IP: Mouse heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19727-245 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
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| WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
|
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| Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
|
Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Ubiquitous; highest levels of expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 27273 Mouse
- Entrez Gene: 89813 Rat
- SwissProt: O70571 Mouse
- SwissProt: O54937 Rat
- Unigene: 235547 Mouse
- Unigene: 11766 Rat
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Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
- FLJ40832 antibody
- mitochondrial antibody
see all
Images
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Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution
Lane 1 : Mouse PDK4 recombinant protein
Lane 2 : Mouse PDK1 recombinant protein
Lane 3 : Mouse PDK2 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.
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![Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-266940-anti-pdk4-antibody-epr19727-245-western-blot.jpg)
Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat muscle lysate
Lane 4 : Rat heart lysate
Lane 5 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2-4: 3 minutes; Lane 5: 1 minutes; Lane 6: 10 seconds.
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![Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-266939-anti-pdk4-antibody-epr19727-245-western-blot.jpg)
Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)Lanes 1-2 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution
Lanes 3-4 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution
Lane 1 : Heart from nonfasting mouse
Lane 2 : Heart from mouse fasting for 72 hours
Lane 3 : Gastrocnemius muscle from nonfasting mouse
Lane 4 : Gastrocnemius muscle from mouse fasting for 72 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression level of PDK4 is increased under the fasting state (PMID: 11723055 and PMID: 16606348).
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![Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-266937-anti-pdk4-antibody-epr19727-245-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] (ab214938)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling PDK4 with ab214938 at 1/60 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-266933-anti-pdk4-antibody-epr19727-245-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)PDK4 was immunoprecipitated from 0.35 mg of mouse heart lysate with ab214938 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab214938 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Mouse heart lysate 10µg (Input).
Lane 2: ab214938 IP in mouse heart lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214938 in mouse heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 0.5 second.
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![Anti-PDK4 antibody [EPR19727-245] (ab214938)](https://www.abcam.com/ps/products/214/ab214938/Images/ab214938-6-benefits-of-recombinant-antibodies.png)
Anti-PDK4 antibody [EPR19727-245] (ab214938)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab214938 has been referenced in 10 publications.
- Shannon CE et al. Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease. Mol Metab 45:101154 (2021). PubMed: 33359401
- Liu B et al. Long non-coding RNA AFAP1-AS1 facilitates ovarian cancer progression by regulating the miR-107/PDK4 axis. J Ovarian Res 14:60 (2021). PubMed: 33926489
- Chien HC et al. PPARd and FOXO1 Mediate Palmitate-Induced Inhibition of Muscle Pyruvate Dehydrogenase Complex and CHO Oxidation, Events Reversed by Electrical Pulse Stimulation. Int J Mol Sci 21:N/A (2020). PubMed: 32824862
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Hsu JW et al. Phthalate exposure causes browning-like effects on adipocytes in vitro and in vivo. Food Chem Toxicol 142:111487 (2020). PubMed: 32522588
- Gao K et al. Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism. Chin Med 15:21 (2020). PubMed: 32158496
- Kaya E et al. Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease. J Clin Med 9:N/A (2020). PubMed: 32276303
- Nasci VL et al. miR-21-5p regulates mitochondrial respiration and lipid content in H9C2 cells. Am J Physiol Heart Circ Physiol 316:H710-H721 (2019). PubMed: 30657727
- Pin F et al. PDK4 drives metabolic alterations and muscle atrophy in cancer cachexia. FASEB J 33:7778-7790 (2019). PubMed: 30894018
- Wu P et al. MicroRNA-29a is involved lipid metabolism dysfunction and insulin resistance in C2C12 myotubes by targeting PPARd. Mol Med Rep 17:8493-8501 (2018). PubMed: 29693165