PE Anti-CD19 antibody [EPR23174-145] (ab306537)
Key features and details
- PE Rabbit monoclonal [EPR23174-145] to CD19
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Related conjugates and formulations
Overview
-
Product name
PE Anti-CD19 antibody [EPR23174-145]
See all CD19 primary antibodies -
Description
PE Rabbit monoclonal [EPR23174-145] to CD19 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: C57 BL/6 Mouse Splenocytes
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23174-145 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab306537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/500.
|
Notes |
---|
Flow Cyt
1/500. |
Target
-
Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12478 Mouse
- SwissProt: P25918 Mouse
- Unigene: 4360 Mouse
-
Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Images
-
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab306537 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab306537) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100μl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab306537 has not yet been referenced specifically in any publications.