Recombinant Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287587-anti-prolactin-prl-antibody-epr18018-31-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18018-31] to Prolactin/PRL
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Prolactin/PRL antibody [EPR18018-31]
See all Prolactin/PRL primary antibodies -
Description
Rabbit monoclonal [EPR18018-31] to Prolactin/PRL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat pituitary tissue lysates. IHC-P: Mouse and rat pituitary tissues. IHC-Fr: Mouse pituitary tissue. IP: Mouse pituitary tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18018-31 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody is not suitable for human species in IHC application. |
|
| IHC-Fr |
1/1000.
Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol. Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa). |
|
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not suitable for human species in IHC application. |
|
IHC-Fr
1/1000. Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol. Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.
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IP
1/30. |
Target
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 5617 Human
- Entrez Gene: 19109 Mouse
- Entrez Gene: 24683 Rat
- Omim: 176760 Human
- SwissProt: P01236 Human
- SwissProt: P06879 Mouse
- SwissProt: P01237 Rat
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Alternative names
- Decidual prolactin antibody
- GHA1 antibody
- Growth hormone A1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID: 24514953). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287585-anti-prolactin-prl-antibody-epr18018-31-western-blot.jpg)
Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/2000 dilution + Human pituitary tissue lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287586-anti-prolactin-prl-antibody-epr18018-31-western-blot.jpg)
Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)All lanes : Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/5000 dilution
Lane 1 : Rat pituitary tissue lysate
Lane 2 : Mouse pituitary tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST
Prolactin expression is generally restricted to the anterior pituitary gland (PMID: 1697858).
Negative control: Mouse kidney, rat heart and C6 cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287588-anti-prolactin-prl-antibody-epr18018-31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID: 24514953). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287589-anti-prolactin-prl-antibody-epr18018-31-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID: 16468032). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-287869-anti-prolactin-prl-antibody-epr18018-31-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse pituitary tissue lysate 10 µg (Input).
Lane 2: ab188229 IP in mouse pituitary tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188229 in mouse pituitary tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)](https://www.abcam.com/ps/products/188/ab188229/Images/ab188229-7-benefits-of-recombinant-antibodies.png)
Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab188229 has been referenced in 1 publication.
- Chen Y et al. Genomic and transcriptomic analysis of pituitary adenomas reveals the impacts of copy number variations on gene expression and clinical prognosis among prolactin-secreting subtype. Aging (Albany NY) 13:1276-1293 (2020). PubMed: 33472173