RecombinantRabMAb

Recombinant Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
  • Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18018-31] to Prolactin/PRL
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

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Carrier Free

Overview

  • Product name

    Anti-Prolactin/PRL antibody [EPR18018-31]
    See all Prolactin/PRL primary antibodies

  • Description

    Rabbit monoclonal [EPR18018-31] to Prolactin/PRL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat pituitary tissue lysates. IHC-P: Mouse and rat pituitary tissues. IHC-Fr: Mouse pituitary tissue. IP: Mouse pituitary tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18018-31

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not suitable for human species in IHC application.
IHC-Fr
1/1000.

Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol.

Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.

 
IP
1/30.
Notes
WB
1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not suitable for human species in IHC application.
IHC-Fr
1/1000.

Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol.

Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.

 
IP
1/30.

Target

  • Relevance

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Decidual prolactin antibody
      • GHA1 antibody
      • Growth hormone A1 antibody
      • Lactogenic hormone antibody
      • Luteotropic hormone antibody
      • Mammotropin antibody
      • PRL antibody
      • Prolactin antibody
      • Prolactin precursor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

      Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID: 24514953). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/2000 dilution + Human pituitary tissue lysate at 10 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      All lanes : Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/5000 dilution

      Lane 1 : Rat pituitary tissue lysate
      Lane 2 : Mouse pituitary tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Rat heart tissue lysate
      Lane 5 : C6 (rat glial tumor cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST

      Prolactin expression is generally restricted to the anterior pituitary gland (PMID: 1697858).

      Negative control: Mouse kidney, rat heart and C6 cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

      Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID: 24514953). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID: 16468032). The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

      Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse pituitary tissue lysate 10 µg (Input). 

      Lane 2: ab188229 IP in mouse pituitary tissue lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188229 in mouse pituitary tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)
      Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab188229? Please let us know so that we can cite the reference in this datasheet.

    ab188229 has been referenced in 1 publication.

    • Chen Y  et al. Genomic and transcriptomic analysis of pituitary adenomas reveals the impacts of copy number variations on gene expression and clinical prognosis among prolactin-secreting subtype. Aging (Albany NY) 13:1276-1293 (2020). PubMed: 33472173

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