For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    quinone-oxidoreductase-antibody-epr10843b-ab154842.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Sensory System Visual system
Share by email
RecombinantRabMAb

Recombinant Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
  • Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)
Primary
Product image
Anti-LRIG3 antibody (ab197989)

View more associated products

Overview

  • Product name

    Anti-Quinone oxidoreductase antibody [EPR10843(B)]
    See all Quinone oxidoreductase primary antibodies
  • Description

    Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver lysate; HepG2 and K562 cell lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10843(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Visual system
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism

Associated products

  • Alternative Versions

    • Anti-Quinone oxidoreductase antibody [EPR10843(B)] - BSA and Azide free (ab249145)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
    • Recombinant Human CRYZL1 protein (ab95503)
  • Recombinant Protein

    • Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
  • Tissue specificity

    Only very low amounts in the lens.
  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q08257 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1429 Human
    • Omim: 123691 Human
    • SwissProt: Q08257 Human
    • Unigene: 83114 Human
    • Alternative names

      • Crystallin zeta (quinone reductase) antibody
      • Crystallin zeta antibody
      • Cryz antibody
      • DKFZp779E0834 antibody
      • FLJ41475 antibody
      • NADPH:quinone reductase antibody
      • QOR_HUMAN antibody
      • Quinone oxidoreductase antibody
      • Zeta crystallin antibody
      • Zeta-crystallin antibody
      see all

    Images

    • Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      All lanes : Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunofluorescence analysis of HepG2 cells, labeling Quinone oxidoreductase using ab154842 at a 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

      Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154842 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
      Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab154842? Please let us know so that we can cite the reference in this datasheet.

    ab154842 has been referenced in 1 publication.

    • Garzón I  et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol 8:681 (2020). PubMed: 32671048

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab154842.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.