Recombinant Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
![Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161441-anti-cryz-antibody-epr10843b-western-blot.jpg "Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Quinone oxidoreductase antibody [EPR10843(B)]
See all Quinone oxidoreductase primary antibodies -
Description
Rabbit monoclonal [EPR10843(B)] to Quinone oxidoreductase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver lysate; HepG2 and K562 cell lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10843(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. -
Tissue specificity
Only very low amounts in the lens. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1429 Human
- Omim: 123691 Human
- SwissProt: Q08257 Human
- Unigene: 83114 Human
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Alternative names
- Crystallin zeta (quinone reductase) antibody
- Crystallin zeta antibody
- Cryz antibody
see all
Images
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Western blot - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)All lanes : Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161440-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161439-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling Quinone oxidoreductase using ab154842 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161438-anti-cryz-antibody-epr10843b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunofluorescence analysis of HepG2 cells, labeling Quinone oxidoreductase using ab154842 at a 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161437-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154842 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161436-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154842 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161435-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154842 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-161434-anti-cryz-antibody-epr10843b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154842 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)](https://www.abcam.com/ps/products/154/ab154842/Images/ab154842-9-benefits-of-recombinant-antibodies.png)
Anti-Quinone oxidoreductase antibody [EPR10843(B)] (ab154842)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab154842 has been referenced in 1 publication.
- Garzón I et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol 8:681 (2020). PubMed: 32671048
