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    rip3-phospho-s227-antibody-epr9627-ab209384.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (49)

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Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
  • Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
  • ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
  • Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
  • Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
  • Suitable for: ELISA, Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-RIP3 (phospho S227) antibody [EPR9627]
    See all RIP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 8 h and then harvested whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9627
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cancer
    • Cell Death
    • Necroptosis

Associated products

  • Alternative Versions

    • Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human RIP3 protein (Tagged) (ab125566)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Dot blot
1/1000.
WB
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
Notes
ELISA
Use at an assay dependent concentration.
Dot blot
1/1000.
WB
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Promotes apoptosis.
  • Tissue specificity

    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9Y572 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11035 Human
    • Omim: 605817 Human
    • SwissProt: Q9Y572 Human
    • Unigene: 268551 Human
    • Alternative names

      • Receptor interacting protein 3 antibody
      • Receptor interacting serine threonine kinase 3 antibody
      • Receptor interacting serine/threonine protein kinase 3 antibody
      • Receptor-interacting protein 3 antibody
      • Receptor-interacting serine/threonine-protein kinase 3 antibody
      • RIP 3 antibody
      • RIP like protein kinase 3 antibody
      • RIP-3 antibody
      • RIP-like protein kinase 3 antibody
      • RIPK 3 antibody
      • RIPK3 antibody
      • RIPK3_HUMAN antibody
      see all

    Images

    • Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilution

      Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysate
      Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 100 seconds


      Blocking/Diluting buffer and concentration 5% NFDM/TBST

    • Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

      Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

      Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.

    • Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

      Lane 1 : Untreated HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 uM z-VAD for 6 h and then harvested whole cell lysate

      Predicted band size: 57 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Image provided by Xiaodong. Wang, National Institute of Biological Sciences, Beijing.

    • Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
      Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (49)

    Publishing research using ab209384? Please let us know so that we can cite the reference in this datasheet.

    ab209384 has been referenced in 49 publications.

    • Roedig J  et al. USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination. EMBO Rep 22:e50163 (2021). PubMed: 33369872
    • Wang KJ  et al. Emodin Induced Necroptosis and Inhibited Glycolysis in the Renal Cancer Cells by Enhancing ROS. Oxid Med Cell Longev 2021:8840590 (2021). PubMed: 33532038
    • Yin Z  et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021). PubMed: 33980862
    • Hu H  et al. RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif. Cell Death Differ 28:251-266 (2021). PubMed: 32737444
    • Zhao W  et al. Dihydrotanshinone I Attenuates Plaque Vulnerability in Apolipoprotein E-Deficient Mice: Role of Receptor-Interacting Protein 3. Antioxid Redox Signal 34:351-363 (2021). PubMed: 32323566
    View all Publications for this product

    Customer reviews and Q&As

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