Recombinant Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
![Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)](https://www.abcam.com/ps/products/209/ab209384/Images/ab209384-394073-anti-rip3-phospho-s227-antibody-epr9627-westernblot-ht-29-human.jpg "Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
- Suitable for: ELISA, Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-RIP3 (phospho S227) antibody [EPR9627]
See all RIP3 primary antibodies -
Description
Rabbit monoclonal [EPR9627] to RIP3 (phospho S227) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 8 h and then harvested whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9627 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| Dot blot |
1/1000.
|
|
| WB |
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Dot blot
1/1000. |
|
WB
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
-
Function
Promotes apoptosis. -
Tissue specificity
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 11035 Human
- Omim: 605817 Human
- SwissProt: Q9Y572 Human
- Unigene: 268551 Human
-
Alternative names
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
Images
-
Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 100 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
-
![Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)](https://www.abcam.com/ps/products/209/ab209384/Images/ab209384-256468-anti-rip3-phospho-s227-antibody-epr9627-dot-blot.jpg)
Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
-
![ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)](https://www.abcam.com/ps/products/209/ab209384/Images/ab209384-272265-anti-rip3-phospho-s227-antibody-epr9627-elisa.jpg)
ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.
-
![Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)](https://www.abcam.com/ps/products/209/ab209384/Images/ab209384-256469-anti-rip3-phospho-s227-antibody-epr9627-western-blot.jpg)
Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
Lane 1 : Untreated HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 uM z-VAD for 6 h and then harvested whole cell lysate
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Image provided by Xiaodong. Wang, National Institute of Biological Sciences, Beijing.
-
![Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)](https://www.abcam.com/ps/products/209/ab209384/Images/ab209384-6-benefits-of-recombinant-antibodies.png)
Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (49)
ab209384 has been referenced in 49 publications.
- Roedig J et al. USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination. EMBO Rep 22:e50163 (2021). PubMed: 33369872
- Wang KJ et al. Emodin Induced Necroptosis and Inhibited Glycolysis in the Renal Cancer Cells by Enhancing ROS. Oxid Med Cell Longev 2021:8840590 (2021). PubMed: 33532038
- Yin Z et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021). PubMed: 33980862
- Hu H et al. RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif. Cell Death Differ 28:251-266 (2021). PubMed: 32737444
- Zhao W et al. Dihydrotanshinone I Attenuates Plaque Vulnerability in Apolipoprotein E-Deficient Mice: Role of Receptor-Interacting Protein 3. Antioxid Redox Signal 34:351-363 (2021). PubMed: 32323566
