Recombinant Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein
- Suitable for: WB, ELISA
Related conjugates and formulations
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric)
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein -
Host species
Human -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419. Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
Database link: P0DTC9 -
Positive control
- ELISA: Human Novel Coronavirus (SARS-CoV-2/ 2020-nCoV) samples. WB: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli.
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General notes
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.
As the Fc region is human, an anti-Human secondary antibody should be used for detection.
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells. -
Clonality
Monoclonal -
Clone number
1A6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/5000. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. -
Alternative names
- 2019-nCoV nucleocapsid protein antibody
- N antibody
- NC antibody
see all
Images
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Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) at 1/1000 dilution + His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng
Secondary
Peroxidase Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution -
Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab272852 has been referenced in 5 publications.
- Khan WH et al. Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19. Int J Biol Macromol 200:428-437 (2022). PubMed: 35051498
- Schmidt N et al. The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol 6:339-353 (2021). PubMed: 33349665
- Klausberger M et al. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests. EBioMedicine 67:103348 (2021). PubMed: 33906067
- Lutomski CA et al. Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies. JACS Au 1:1147-1157 (2021). PubMed: 34462738
- Allison G et al. A Fabry-Pérot cavity coupled surface plasmon photodiode for electrical biomolecular sensing. Nat Commun 12:6483 (2021). PubMed: 34759292