For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    rabbit-monoclonal-epr24334-118-to-sars-cov-2-nucleocapsid-protein-ab271180.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
Share by email
RecombinantRabMAb

Recombinant Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

  • Datasheet
  • SDS
Reviews (3) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • Immunoprecipitation - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein
  • Suitable for: ELISA, IP, IHC-P, Flow Cyt (Intra), WB, ICC/IF

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

You may also be interested in

Primary
Product image
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
Protein
Product image
Recombinant Hantavirus Dobrava, strain Slovenia, nucleocapsid protein (ab74556)

View more associated products

Overview

  • Product name

    Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein
    See all SARS-CoV-2 nucleocapsid protein primary antibodies
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, IP, IHC-P, Flow Cyt (Intra), WB, ICC/IFmore details
  • Species reactivity

    Reacts with: SARS-CoV-2
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with myc-His-tag® SARS-CoV-2 Nucleocapsid protein; SARS-CoV-2 Nucleocapsid Recombinant Protein; SARS-CoV-2 infected Vero; ab273530; ab272107 IHC-P: HEK-293T transfected with myc-His-tag® SARS-CoV-2. ICC/IF: 293T transfected with myc-His-tag® SARS-CoV-2; Vero cells after infecting with SARS-CoV-2 virus for 48 h. Flow Cyt (intra): HEK-293T transfected with myc-His-tag® SARS-CoV-2. IP: HEK-293T transfected with myc-His-tag® SARS-CoV-2.
  • General notes

    This antibody is developed with the collaboration of Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR24334-118
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

Associated products

  • Alternative Versions

    • Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Dyes/Markers

    • Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.

Use at 31.25 ng /ml

IP
1/30.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
WB (2)
1/1000.
ICC/IF (1)
1/1000.
Notes
ELISA
Use at an assay dependent concentration.

Use at 31.25 ng /ml

IP
1/30.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
WB
1/1000.
ICC/IF
1/1000.

Target

  • Relevance

    Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
  • Alternative names

    • 2019-nCoV nucleocapsid protein antibody
    • N antibody
    • NC antibody
    • Nucleoprotein antibody
    • Protein N antibody
    see all

Images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    All lanes : Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney) transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293T (human embryonic kidney) transfected with MERS Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 51 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time 7.75 seconds

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    All lanes : Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution

    Lane 1 : SARS-CoV-2 Nucleocapsid Recombinant Protein at 0.1 µg
    Lane 2 : SARS-CoV-2 infected Vero for 48 hours
    Lane 3 : Uninfected Vero

    Observed band size: 51 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    All lanes : Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution

    Lanes 1 & 4 : Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
    Lanes 2 & 5 : Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829)
    Lanes 3 & 6 : Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107)

    Lysates/proteins at 0.1 µg per lane.

    Performed under reducing conditions.

    Observed band size: 51 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Green - ab271180 observed at 51 kDa. Lanes 4 - 6: Red - loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 51 kDa.

    ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab271180 and ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Samples

    Lanes 1 & 3: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab273530) (0.2 µg)

    Lanes 2 & 4: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) (0.2 µg)

     

    Green: Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

    Red: Mouse monoclonal [HIS.H8] to 6X His tag® (ab18184)

     

    Lanes 1 & 2: Green - ab271180 observed at 50 kDa. 

    Lanes 3 & 4: Red - loading control Mouse anti-6x HisTag observed at 50 kDa.

     

    ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag (ab18184) overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Immunohistochemical analysis of paraffin-embedded Human lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on human lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel A) 
    HEK-293T transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel B) HEK-293T transfected with MERS Nucleocapsid protein expression vector containing a myc-His-tag® (Panel C) HEK-293T transfected with control vector containing a myc-His-tag® (Panel D) with 271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on (A) HEK-293T transfected with a SARS-CoV-2 Nucleocapsid protein expression vector is observed. No staining on (B) HEK-293T transfected with a SARS-CoV-1 Nucleocapsid protein expression vector, (C) HEK-293T transfected with MERS Nucleocapsid protein expression vector and (D) HEK-293T transfected with empty vector. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Immunohistochemical analysis of paraffin-embedded Mouse lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Mouse lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Immunohistochemical analysis of paraffin-embedded Rat lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Rat lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
    Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector.

     

  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
    Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized Vero cells labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/500 dilution. Fluorescent image showing positve staining in Vero cells after infecting with SARS-CoV-2 virus for 48 h. Nuclear counter stain DAPI. 
    Two indenpendent optical fields were captured for imaging at each given antibody dilution.

    This piece of data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine

  • Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
    Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing no staining in 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein, which are negative controls for SARS-CoV-2.

     

  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression construct  labelling SARS-CoV-2 with ab271180 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-1 Nucleocapsid protein expression construct (Left)/ HEK-293T (human embryonic kidney) transfected with myc-tagged MERS Nucleocapsid protein expression construct (Right). 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    Immunoprecipitation - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    SARS-CoV-2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate 5 µg with ab271180 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate 5 μg

    Lane 2: ab271180 IP in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271180 in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

     

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)
    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180)

    Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.

    Primary antibody (ab271180) concentration range 0.045-100 ng /ml.

    Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) used at 1/2000 dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab271180? Please let us know so that we can cite the reference in this datasheet.

ab271180 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
SARS-CoV-2 Cell (human ACE-2 expressing A549 cells)
Permeabilization
Yes - BD Permwash
Specification
human ACE-2 expressing A549 cells
Blocking step
BD Permwash as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
10% formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tessa Prince

Verified customer

Submitted May 13 2021

Western blot abreview for Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Vero E6)
Gel Running Conditions
Reduced Denaturing (NuPAGE Bis-Tris 4-12%)
Loading amount
5 µg
Treatment
SARS CoV2 variants 24hpi
Specification
Vero E6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Stuart Armstrong

Verified customer

Submitted May 12 2021

Western blot abreview for Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Caco-2 human colorectal adenocarcinoma cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Caco-2 human colorectal adenocarcinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Nicola Goodman

Verified customer

Submitted Apr 30 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.