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RecombinantRabMAb

Recombinant Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

Reviews (1) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
  • Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22697-17] to Slow Skeletal Myosin Heavy chain
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17]
    See all Slow Skeletal Myosin Heavy chain primary antibodies

  • Description

    Rabbit monoclonal [EPR22697-17] to Slow Skeletal Myosin Heavy chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skeletal muscle, cardiac muscle and spleen tissue; Mouse and rat cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22697-17

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab234431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
IHC-Fr
1/200.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
IHC-Fr
1/200.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Application notes
Is unsuitable for ICC/IF,IP or WB.

Target

  • Function

    Muscle contraction.

  • Tissue specificity

    Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

  • Involvement in disease

    Defects in MYH7 are the cause of cardiomyopathy familial hypertrophic type 1 (CMH1) [MIM:192600]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYH7 are the cause of myopathy myosin storage (MYOMS) [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers.
    Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM) [MIM:181430]; also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm.
    Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S) [MIM:613426]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in MYH7 are the cause of myopathy distal type 1 (MPD1) [MIM:160500]. MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.

  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Beta myosin heavy chain antibody
    • cardiac muscle beta isoform antibody
    • CMD1S antibody
    • CMH1 antibody
    • MPD1 antibody
    • MYH7 antibody
    • MYH7_HUMAN antibody
    • Myhc slow antibody
    • MyHC-beta antibody
    • MyHC-slow antibody
    • MYHCB antibody
    • Myopathy, distal 1 antibody
    • Myosin heavy chain (AA 1-96) antibody
    • Myosin heavy chain 7 antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain slow isoform antibody
    • Myosin heavy chain, cardiac muscle beta isoform antibody
    • Myosin, heavy chain 7, cardiac muscle, beta antibody
    • Myosin, heavy polypeptide 7, cardiac muscle, beta antibody
    • Myosin-7 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.7A antibody
    • SPMD antibody
    • SPMM antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human skeletal muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized rat skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.  Cytoplasmic staining on the rat skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. 

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.  Cytoplasmic staining on the mouse skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative staining on the smooth muscle in the human spleen (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the rat cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the mouse cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)
    Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab234431? Please let us know so that we can cite the reference in this datasheet.

ab234431 has been referenced in 1 publication.

  • Sachdev U  et al. Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquine. Mol Med 26:69 (2020). PubMed: 32641037

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Spalax (blind mole rat) Tissue (muscle tissue (Paraffin sections))
Antigen retrieval step
None
Permeabilization
No
Specification
muscle tissue (Paraffin sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Dr. Lital Sharvit

Verified customer

Submitted Jun 04 2021

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