Recombinant Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-250241-anti-stat5-phospho-y694-antibody-e208-western-blot.jpg "Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E208] to STAT5 (phospho Y694)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STAT5 (phospho Y694) antibody [E208]
See all STAT5 primary antibodies -
Description
Rabbit monoclonal [E208] to STAT5 (phospho Y694) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E208 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
| IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
see all -
Alternative names
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
Images
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Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (purified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Epidermal growth factor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-250259-anti-stat5-phospho-y694-antibody-e208-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-261378-anti-stat5-phospho-y694-antibody-e208-elisa.jpg)
ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)This image is courtesy of Hanna DrejaSerially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
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![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-44577-anti-stat5-phospho-y694-antibody-e208-western-blot.gif)
Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (unpurified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lane 3 : A431 cell lysate, treated with phosphatase
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-44576-anti-stat5-phospho-y694-antibody-e208-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.
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![Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.com/ps/products/32/ab32364/Images/ab32364-6-benefits-of-recombinant-antibodies.png)
Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (48)
ab32364 has been referenced in 48 publications.
- Gao H et al. Characterization of murine mammary stem/progenitor cells in a D-galactose-induced aging model. Aging (Albany NY) 13:11762-11773 (2021). PubMed: 33878032
- Li R et al. Identification and In Vitro Functional Verification of Two Novel Mutations of GHR Gene in the Chinese Children with Laron Syndrome. Front Endocrinol (Lausanne) 12:605736 (2021). PubMed: 33912130
- Mäkinen A et al. IGF2BP3 Associates with Proliferative Phenotype and Prognostic Features in B-Cell Acute Lymphoblastic Leukemia. Cancers (Basel) 13:N/A (2021). PubMed: 33805930
- Zhang J et al. Hydrogen sulfide is a regulator of mammary gland development in prepubescent female mice. Mol Med Rep 22:4061-4069 (2020). PubMed: 33000185
- Bi CS et al. Calcitriol inhibits osteoclastogenesis in an inflammatory environment by changing the proportion and function of T helper cell subsets (Th2/Th17). Cell Prolif 53:e12827 (2020). PubMed: 32406154