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  1. Link

    taf15-antibody-epr9197b-ab134916.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIID
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RecombinantRabMAb

Recombinant Anti-TAF15 antibody [EPR9197(B)] (ab134916)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (7)

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Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9197(B)] to TAF15
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-TAF15 antibody [EPR9197(B)]
    See all TAF15 primary antibodies
  • Description

    Rabbit monoclonal [EPR9197(B)] to TAF15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TAF15 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q92804

  • Positive control

    • HeLa, 293T, Caco 2, and Human fetal kidney lysates; Human urinary bladder carcinoma tissue; HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9197(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIID
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TAFs

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-TAF15 antibody [EPR9197(B)] (ab205775)
    • Alexa Fluor® 647 Anti-TAF15 antibody [EPR9197(B)] (ab205776)
    • Anti-TAF15 antibody [EPR9197(B)] - BSA and Azide free (ab235079)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human TAF15 protein (ab174418)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.

Target

  • Function

    RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • Tissue specificity

    Ubiquitous. Observed in all fetal and adult tissues.
  • Involvement in disease

    Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • Sequence similarities

    Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Target information above from: UniProt accession Q92804 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8148 Human
    • Entrez Gene: 70439 Mouse
    • Entrez Gene: 287571 Rat
    • Omim: 601574 Human
    • SwissProt: Q92804 Human
    • Unigene: 402752 Human
    • Alternative names

      • 68 kDa TATA-binding protein-associated factor antibody
      • Npl3 antibody
      • RBP56 antibody
      • RBP56_HUMAN antibody
      • RNA binding protein 56 antibody
      • RNA-binding protein 56 antibody
      • TAF antibody
      • TAF(II)68 antibody
      • TAF15 antibody
      • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
      • TAF2N antibody
      • TAFII68 antibody
      • TATA-binding protein-associated factor 2N antibody
      see all

    Images

    • Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      All lanes : Anti-TAF15 antibody [EPR9197(B)] (ab134916) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 62 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      ab134916 staining TAF15 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Overlay histogram showing HeLa cells stained with ab134916 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134916, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Immunohistochemical analysis of TAF15 in paraffin embedded Human urinary bladder carcinoma tissue, using ab134916 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunofluorescent analysis of TAF15 in HeLa cells, using ab134916 at a 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Immunohistochemical analysis using ab134916 showing positive staining in Normal human uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Anti-TAF15 antibody [EPR9197(B)] (ab134916)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab134916? Please let us know so that we can cite the reference in this datasheet.

    ab134916 has been referenced in 7 publications.

    • Yang P  et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell 181:325-345.e28 (2020). PubMed: 32302571
    • Chen L  et al. USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1. Cell Death Dis 10:296 (2019). PubMed: 30931936
    • Tischbein M  et al. The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing. J Biol Chem 294:10194-10210 (2019). PubMed: 31092554
    • Xiao R  et al. Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell 178:107-121.e18 (2019). PubMed: 31251911
    • Hock EM  et al. Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24:987-1000.e7 (2018). PubMed: 30044993
    • Gasperini L  et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839
    • Izhar L  et al. A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors. Cell Rep 11:1486-500 (2015). PubMed: 26004182

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TAF15 antibody [EPR9197(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Permeabilization
    Yes - denature of protein binding surface, using 10mM Natirum Glrate +0.02% tween 20 at 99 degree 20min
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 18 2013

    ChIP abreview for Anti-TAF15 antibody [EPR9197(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Negative control
    IgG
    Specification
    primary hepatoctyes
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Positive control
    Rad21 on IGF2 DMR
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 16 2013

    Immunoprecipitation abreview for Anti-TAF15 antibody [EPR9197(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Hep3B)
    Total protein in input
    40000 cells
    Immuno-precipitation step
    Protein A
    Specification
    Hep3B
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 19 2013

    Western blot abreview for Anti-TAF15 antibody [EPR9197(B)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 19 2013

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