Recombinant Anti-TAF15 antibody [EPR9197(B)] (ab134916)
![Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-149058-anti-taf15-antibody-epr9197b-western-blot.jpg "Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9197(B)] to TAF15
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TAF15 antibody [EPR9197(B)]
See all TAF15 primary antibodies -
Description
Rabbit monoclonal [EPR9197(B)] to TAF15 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human TAF15 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q92804 -
Positive control
- HeLa, 293T, Caco 2, and Human fetal kidney lysates; Human urinary bladder carcinoma tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9197(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF | (1) |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 62 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). -
Tissue specificity
Ubiquitous. Observed in all fetal and adult tissues. -
Involvement in disease
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. -
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsDimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8148 Human
- Entrez Gene: 70439 Mouse
- Entrez Gene: 287571 Rat
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
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Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
see all
Images
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Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)All lanes : Anti-TAF15 antibody [EPR9197(B)] (ab134916) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 62 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-261417-anti-taf15-antibody-epr9197b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)ab134916 staining TAF15 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1: PBS only.
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![Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-215929-anti-taf15-antibody-epr9197b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)Overlay histogram showing HeLa cells stained with ab134916 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134916, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-149057-anti-taf15-antibody-epr9197b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)Immunohistochemical analysis of TAF15 in paraffin embedded Human urinary bladder carcinoma tissue, using ab134916 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-149056-anti-taf15-antibody-epr9197b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)Immunofluorescent analysis of TAF15 in HeLa cells, using ab134916 at a 1/250 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-149055-anti-taf15-antibody-epr9197b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)Immunohistochemical analysis using ab134916 showing positive staining in Normal human uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TAF15 antibody [EPR9197(B)] (ab134916)](https://www.abcam.com/ps/products/134/ab134916/Images/ab134916-10-benefits-of-recombinant-antibodies.png)
Anti-TAF15 antibody [EPR9197(B)] (ab134916)
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab134916 has been referenced in 7 publications.
- Yang P et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell 181:325-345.e28 (2020). PubMed: 32302571
- Chen L et al. USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1. Cell Death Dis 10:296 (2019). PubMed: 30931936
- Tischbein M et al. The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing. J Biol Chem 294:10194-10210 (2019). PubMed: 31092554
- Xiao R et al. Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell 178:107-121.e18 (2019). PubMed: 31251911
- Hock EM et al. Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24:987-1000.e7 (2018). PubMed: 30044993
- Gasperini L et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839
- Izhar L et al. A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors. Cell Rep 11:1486-500 (2015). PubMed: 26004182
