Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Reviews (4) Submit a question References (7)
Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
  • Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9197(B)] to TAF15
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

Overview

  • Product name

    Anti-TAF15 antibody [EPR9197(B)]
    See all TAF15 primary antibodies

  • Description

    Rabbit monoclonal [EPR9197(B)] to TAF15

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human TAF15 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q92804

  • Positive control

    • HeLa, 293T, Caco 2, and Human fetal kidney lysates; Human urinary bladder carcinoma tissue; HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.

Target

  • Function

    RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).

  • Tissue specificity

    Ubiquitous. Observed in all fetal and adult tissues.

  • Involvement in disease

    Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.

  • Sequence similarities

    Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.

  • Post-translational
    modifications

    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • 68 kDa TATA-binding protein-associated factor antibody
      • Npl3 antibody
      • RBP56 antibody
      • RBP56_HUMAN antibody
      • RNA binding protein 56 antibody
      • RNA-binding protein 56 antibody
      • TAF antibody
      • TAF(II)68 antibody
      • TAF15 antibody
      • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
      • TAF2N antibody
      • TAFII68 antibody
      • TATA-binding protein-associated factor 2N antibody
      see all

    Images

    • Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      All lanes : Anti-TAF15 antibody [EPR9197(B)] (ab134916) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 62 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      ab134916 staining TAF15 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Overlay histogram showing HeLa cells stained with ab134916 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134916, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Immunohistochemical analysis of TAF15 in paraffin embedded Human urinary bladder carcinoma tissue, using ab134916 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunofluorescent analysis of TAF15 in HeLa cells, using ab134916 at a 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

      Immunohistochemical analysis using ab134916 showing positive staining in Normal human uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TAF15 antibody [EPR9197(B)] (ab134916)
      Anti-TAF15 antibody [EPR9197(B)] (ab134916)

    References (7)

    Publishing research using ab134916? Please let us know so that we can cite the reference in this datasheet.

    ab134916 has been referenced in 7 publications.

    • Yang P  et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell 181:325-345.e28 (2020). PubMed: 32302571
    • Chen L  et al. USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1. Cell Death Dis 10:296 (2019). PubMed: 30931936
    • Tischbein M  et al. The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing. J Biol Chem 294:10194-10210 (2019). PubMed: 31092554
    • Xiao R  et al. Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell 178:107-121.e18 (2019). PubMed: 31251911
    • Hock EM  et al. Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24:987-1000.e7 (2018). PubMed: 30044993
    • Gasperini L  et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839
    • Izhar L  et al. A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors. Cell Rep 11:1486-500 (2015). PubMed: 26004182

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TAF15 antibody [EPR9197(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Permeabilization
    Yes - denature of protein binding surface, using 10mM Natirum Glrate +0.02% tween 20 at 99 degree 20min
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 18 2013

    ChIP abreview for Anti-TAF15 antibody [EPR9197(B)]

     Excellent
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Negative control
    IgG
    Specification
    primary hepatoctyes
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Positive control
    Rad21 on IGF2 DMR
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 16 2013

    Immunoprecipitation abreview for Anti-TAF15 antibody [EPR9197(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Hep3B)
    Total protein in input
    40000 cells
    Immuno-precipitation step
    Protein A
    Specification
    Hep3B
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 19 2013

    Western blot abreview for Anti-TAF15 antibody [EPR9197(B)]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com