Name: Anti-TAF15 antibody [EPR9197(B)]
Brand: RabMAb
SKU: ab134916
Rating: 5 (4 reviews)

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Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9197(B)] to TAF15
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-TAF15 antibody [EPR9197(B)]
See all TAF15 primary antibodies
Description

Rabbit monoclonal [EPR9197(B)] to TAF15
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide within Human TAF15 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q92804
Positive control
- HeLa, 293T, Caco 2, and Human fetal kidney lysates; Human urinary bladder carcinoma tissue; HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9197(B)
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant Human TAF15 protein (ab174418)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(1)	1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF	(1)	1/250 - 1/500.

Notes
Flow Cyt (Intra) 1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.

Function

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Tissue specificity

Ubiquitous. Observed in all fetal and adult tissues.
Involvement in disease

Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similarities

Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications

Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization

Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Target information above from: UniProt accession Q92804 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8148 Human
- Entrez Gene: 70439 Mouse
- Entrez Gene: 287571 Rat
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
- RBP56_HUMAN antibody
- RNA binding protein 56 antibody
- RNA-binding protein 56 antibody
- TAF antibody
- TAF(II)68 antibody
- TAF15 antibody
- TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
- TAF2N antibody
- TAFII68 antibody
- TATA-binding protein-associated factor 2N antibody
see all

Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

All lanes : Anti-TAF15 antibody [EPR9197(B)] (ab134916) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

ab134916 staining TAF15 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1: PBS only.
Flow Cytometry (Intracellular) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Overlay histogram showing HeLa cells stained with ab134916 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134916, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Immunohistochemical analysis of TAF15 in paraffin embedded Human urinary bladder carcinoma tissue, using ab134916 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Immunofluorescent analysis of TAF15 in HeLa cells, using ab134916 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Immunohistochemical analysis using ab134916 showing positive staining in Normal human uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab134916? Please let us know so that we can cite the reference in this datasheet.

ab134916 has been referenced in 5 publications.

Chen L et al. USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1. Cell Death Dis 10:296 (2019). PubMed: 30931936
Tischbein M et al. The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing. J Biol Chem 294:10194-10210 (2019). PubMed: 31092554
Hock EM et al. Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24:987-1000.e7 (2018). PubMed: 30044993
Gasperini L et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839
Izhar L et al. A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors. Cell Rep 11:1486-500 (2015). PubMed: 26004182

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Sample

Mouse Cell (primary hepatoctyes)

Specification

primary hepatoctyes

Permeabilization

Yes - denature of protein binding surface, using 10mM Natirum Glrate +0.02% tween 20 at 99 degree 20min

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 18 2013

Application

ChIP

Sample

Mouse Cell lysate - whole cell (primary hepatoctyes)

Negative control

IgG

Specification

primary hepatoctyes

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde

Positive control

Rad21 on IGF2 DMR

Abcam user community

Verified customer

Submitted Sep 16 2013

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (Hep3B)

Total protein in input

40000 cells

Immuno-precipitation step

Protein A

Specification

Hep3B

Abcam user community

Verified customer

Submitted Aug 19 2013

Application

Western blot

Loading amount

30 µg

Gel Running Conditions

Non-reduced Denaturing (10%)

Sample

Mouse Cell lysate - whole cell (primary hepatoctyes)

Specification

primary hepatoctyes

Blocking step

I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (5)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-TAF15 antibody [EPR9197(B)]

ChIP abreview for Anti-TAF15 antibody [EPR9197(B)]

Immunoprecipitation abreview for Anti-TAF15 antibody [EPR9197(B)]

Western blot abreview for Anti-TAF15 antibody [EPR9197(B)]

Post-translational
modifications