Recombinant Anti-TGIF antibody [EP637Y] (ab52955)
![Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)](https://www.abcam.com/ps/products/52/ab52955/Images/ab52955-314348-anti-tgif-antibody-ep637y-immunoprecipitation.jpg "Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP637Y] to TGIF
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TGIF antibody [EP637Y]
See all TGIF primary antibodies -
Description
Rabbit monoclonal [EP637Y] to TGIF -
Host species
Rabbit -
Specificity
ab52955 detects both phosphorylated and unphosphorylated TGIF. -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TGIF aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and K562 cell lysate. IP: HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP637Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).
For unpurified use at 1/20000 |
|
| IP |
1/100.
For unpurified use at 1/30 |
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa). For unpurified use at 1/20000 |
|
IP
1/100. For unpurified use at 1/30 |
Target
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Function
Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. -
Involvement in disease
Holoprosencephaly 4 -
Sequence similarities
Belongs to the TALE/TGIF homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7050 Human
- Omim: 602630 Human
- SwissProt: Q15583 Human
- Unigene: 373550 Human
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Alternative names
- 5' TG 3' interacting factor antibody
- 5''-TG-3''-interacting factor 1 antibody
- Homeobox protein TGIF antibody
see all
Images
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Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)
ab52955 (purified) at 1:100 dilution (2µg) immunoprecipitating TGIF in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52955 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-TGIF antibody [EP637Y] (ab52955)](https://www.abcam.com/ps/products/52/ab52955/Images/ab52955-314287-anti-tgif-antibody-ep637y-western-blot.jpg)
Western blot - Anti-TGIF antibody [EP637Y] (ab52955)Anti-TGIF antibody [EP637Y] (ab52955) at 0.4 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-TGIF antibody [EP637Y] (ab52955)](https://www.abcam.com/ps/products/52/ab52955/Images/ab52955-65492-anti-tgif-antibody-ep637y-western-blot.gif)
Western blot - Anti-TGIF antibody [EP637Y] (ab52955)Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution (Unpurifed) + K562 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
![Anti-TGIF antibody [EP637Y] (ab52955)](https://www.abcam.com/ps/products/52/ab52955/Images/ab52955-4-benefits-of-recombinant-antibodies.png)
Anti-TGIF antibody [EP637Y] (ab52955)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab52955 has been referenced in 6 publications.
- Zhang J et al. TGIF1 Knockdown Inhibits the Proliferation and Invasion of Gastric Cancer via AKT Signaling Pathway. Cancer Manag Res 13:2603-2612 (2021). PubMed: 33776478
- Haider MT et al. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment. Breast Cancer Res 22:34 (2020). PubMed: 32272947
- Zhang T et al. Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma. Oncogenesis 9:92 (2020). PubMed: 33070167
- Liu X et al. The Chinese Medicine, Shezhi Huangling Decoction, Inhibits the Growth and Metastasis of Glioma Cells via the Regulation of miR-1298-5p/TGIF1 Axis. Cancer Manag Res 12:5677-5687 (2020). PubMed: 32765071
- Saito H et al. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone. Nat Commun 10:1354 (2019). PubMed: 30902975
- Yen-Ping Ho J et al. Transforming growth interacting factor expression in leiomyoma compared with myometrium. Fertil Steril 94:1078-1083 (2010). WB . PubMed: 19524896