RecombinantRabMAb

Recombinant Anti-TGIF antibody [EP637Y] (ab52955)

Submit a review Q&A (1)References (5)
Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)
  • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
  • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
  • Anti-TGIF antibody [EP637Y] (ab52955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP637Y] to TGIF
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TGIF antibody [EP637Y]
    See all TGIF primary antibodies

  • Description

    Rabbit monoclonal [EP637Y] to TGIF

  • Host species

    Rabbit

  • Specificity

    ab52955 detects both phosphorylated and unphosphorylated TGIF.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human TGIF aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and K562 cell lysate. IP: HeLa whole cell lysate (ab150035)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).

For unpurified use at 1/20000
IP
1/100.

For unpurified use at 1/30
Notes
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).

For unpurified use at 1/20000
IP
1/100.

For unpurified use at 1/30
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.

  • Involvement in disease

    Holoprosencephaly 4

  • Sequence similarities

    Belongs to the TALE/TGIF homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5' TG 3' interacting factor antibody
      • 5''-TG-3''-interacting factor 1 antibody
      • Homeobox protein TGIF antibody
      • Homeobox protein TGIF1 antibody
      • HPE4 antibody
      • MGC39747 antibody
      • MGC5066 antibody
      • TALE homeobox TG-interacting factor antibody
      • TG interacting factor isoform c antibody
      • TG-interacting factor antibody
      • TGFB induced factor antibody
      • TGFB-induced factor homeobox 1 antibody
      • TGIF1 antibody
      • TGIF1_HUMAN antibody
      • transforming growth factor-beta-induced factor antibody
      see all

    Images

    • Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)
      Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)

      ab52955 (purified) at 1:100 dilution (2µg) immunoprecipitating TGIF in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab52955 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
      Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
      Anti-TGIF antibody [EP637Y] (ab52955) at 0.4 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
      Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
      Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution (Unpurifed) + K562 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?

    • Anti-TGIF antibody [EP637Y] (ab52955)
      Anti-TGIF antibody [EP637Y] (ab52955)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab52955? Please let us know so that we can cite the reference in this datasheet.

    ab52955 has been referenced in 5 publications.

    • Haider MT  et al. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment. Breast Cancer Res 22:34 (2020). PubMed: 32272947
    • Zhang T  et al. Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma. Oncogenesis 9:92 (2020). PubMed: 33070167
    • Liu X  et al. The Chinese Medicine, Shezhi Huangling Decoction, Inhibits the Growth and Metastasis of Glioma Cells via the Regulation of miR-1298-5p/TGIF1 Axis. Cancer Manag Res 12:5677-5687 (2020). PubMed: 32765071
    • Saito H  et al. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone. Nat Commun 10:1354 (2019). PubMed: 30902975
    • Yen-Ping Ho J  et al. Transforming growth interacting factor expression in leiomyoma compared with myometrium. Fertil Steril 94:1078-1083 (2010). WB . PubMed: 19524896

    Customer reviews and Q&As

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    Question

    WB with human samples
    no signal, but not even pos. crtl (stimulated cells) gave no signal
    other antibodies worked and actin was detected
    Ab: 1/500, 1/50
    20 ug protein loaded
    block w/ Odyssee blocking buffer
    secondary: Cy3, is OK
    transfer: worked, other proteins detected

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    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry these 3 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements with the order number xxx for ab50884 and ab78421, and the order number xxx for ab118402 (backordered). The estimated delivery date for the latter product is xxx. I hope this is OK.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

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