Recombinant Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19853] to TIFA (phospho T9) - BSA and Azide free
- Suitable for: IP, Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free
See all TIFA primary antibodies -
Description
Rabbit monoclonal [EPR19853] to TIFA (phospho T9) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate.
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General notes
ab236848 is the carrier-free version of ab214815.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19853 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. -
Sequence similarities
Contains 1 FHA domain. - Information by UniProt
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Database links
- Entrez Gene: 92610 Human
- Omim: 609028 Human
- SwissProt: Q96CG3 Human
- Unigene: 310640 Human
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Alternative names
- Putative MAPK activating protein PM14 antibody
- Putative MAPK-activating protein PM14 antibody
- Putative NF kappa B activating protein 20 antibody
see all
Images
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All lanes : Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) transfected with 3×Flag-tagged TIFA expression vector for 28 hours, whole cell lysate
Lane 2 : HeLa transfected with 3×Flag-tagged TIFA T9A mutant expression vector for 28 hours, whole cell lysate
Lane 3 : HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
Lane 4 : HeLa transfected with 3×Flag-tagged TIFAT9A mutant expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 21 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% milk/TBST.
The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
S. flexneri infection induces an innate immune response (PMID: 28222186).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab214815).
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TIFA (phospho T9) was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab214815 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214815 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate 10 µg (Input).
Lane 2: ab214815 IP in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214815 in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (-).Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).
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Dot blot analysis of TIFA (phospho T9) labeled with ab214815 at 1/1000 dilution.
Lane 1: TIFA (phospho T9) peptide (aa3-12).
Lane 2: TIFA (phospho T9) peptide (aa5-15).
Lane 3: TIFA non-phospho peptide (aa3-15).
Secondary used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab236848 has not yet been referenced specifically in any publications.