Secondary antibodies
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Over 1800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.
Features
Secondary antibody selection guide
Our guide will help you choose the most appropriate secondary antibody for your application.
IRDye® conjugated secondary antibodies
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Direct vs indirect IF
Understand the difference between direct and indirect methods for immunofluorescence.
Alexa Fluor® secondary antibodies
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 conjugated antibodies
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
HRP polymer secondaries
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
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Secondary antibodies optimized for co-IP western blotting
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Can you provide the immunogen sequence?
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Indirect flow cytometry protocol
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ICC/IF protocol
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Immunohistochemistry (IHC): the complete guide
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Hints and tips for fluorescent western blotting
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Benefits and Limitations of Primary and Secondary Antibody Conjugates
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Direct vs indirect immunofluorescence
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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
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IRDye® conjugated secondary antibodies
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Learn about cell imaging techniques through our protocol and webinar series
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Alexa Fluor® conjugated secondaries ideal for multicolor analysis
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Conjugate selection guide for secondary antibodies
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Secondaries you can trust
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Double and triple immunostaining tips.
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Fluorochrome chart – a complete guide
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Anti-mouse IgG for IP (HRP) (ab131368)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
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Goat Anti-Human IgG Fc (HRP) (ab97225)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
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Our guide to secondary antibody selection
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Validated Alexa Fluor secondary antibodies for guaranteed performance
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Goat Anti-Mouse IgG H&L (FITC) (ab6785)
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Why choosing the right secondary antibody matters?
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Multiplexing in fluorescent western blotting
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Alexa Fluor® 488-conjugated antibodies | Abcam
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