Secondary antibodies
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Over 1800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.
Features
Secondary antibody selection guide
Our guide will help you choose the most appropriate secondary antibody for your application.
IRDye® conjugated secondary antibodies
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Direct vs indirect IF
Understand the difference between direct and indirect methods for immunofluorescence.
Alexa Fluor® secondary antibodies
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 conjugated antibodies
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
HRP polymer secondaries
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
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Comprehensive guide to Human CD antigens
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Thoroughly validated directly conjugated antibodies
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Mounting media and sera for imaging
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Validated Alexa Fluor secondary antibodies for guaranteed performance
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Secondary antibodies optimized for co-IP western blotting
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Indirect flow cytometry protocol
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A guide to fluorochromes poster
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How we validate our antibodies
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T cells: basic immunophenotyping
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B cells: Basic immunophenotyping
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Fluorochrome chart – a complete guide
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Direct vs indirect immunofluorescence
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Understanding fluorophores
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Advanced Immunoprecipitation
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Principles and Practice of Confocal Microscopy in Life Sciences
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Immunoprecipitation protocol
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Alexa Fluor® 488-conjugated antibodies | Abcam
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Double and triple immunostaining tips.
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ICC/IF protocol
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Immunohistochemistry (IHC): the complete guide
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Why choosing the right secondary antibody matters?
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Alexa Fluor® conjugated secondaries ideal for multicolor analysis
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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
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Alexa Fluor conjugated secondary antibodies - FAQs
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Fluorescent western blot Alexa Fluor® secondaries
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Conjugate selection guide for secondary antibodies
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Flow cytometric analysis of extracellular vesicles
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Detect low-abundance proteins with biotinylated antibodies
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Procedure for detection of phosphorylated proteins in western blot
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Our guide to secondary antibody selection
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Secondaries you can trust
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Learn about cell imaging techniques through our protocol and webinar series
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Hints and tips for fluorescent western blotting
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IRDye® conjugated secondary antibodies
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Multiplexing in fluorescent western blotting
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Can you provide the immunogen sequence?
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Benefits and Limitations of Primary and Secondary Antibody Conjugates
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Anti-mouse IgG for IP (HRP) (ab131368)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
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Goat Anti-Human IgG Fc (HRP) (ab97225)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
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Goat Anti-Mouse IgG H&L (FITC) (ab6785)
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