Secondary antibodies

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Over 1800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.

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Features

Secondary antibody selection guide

Our guide will help you choose the most appropriate secondary antibody for your application.

IRDye® conjugated secondary antibodies

Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.

Alexa Fluor® secondary antibodies

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Alexa Fluor® 488 conjugated antibodies

Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.

HRP polymer secondaries

Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).

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To make sure you get the faster results in immunostaining with your primary antibody, get the tested secondary antibody too.

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Direct vs indirect immunofluorescence

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Comprehensive guide to Human CD antigens

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Our guide to secondary antibody selection

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Secondaries you can trust

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Learn about cell imaging techniques through our protocol and webinar series

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Mounting media and sera for imaging

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Double and triple immunostaining tips.

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Validated Alexa Fluor secondary antibodies for guaranteed performance

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Pre-adsorbed secondary antibodies - minimize non-specific binding and high background staining

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Find out how to use IRDye® secondary antibodies, with abcam Fluorescent WB protocol.

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Hints and tips for fluorescent western blotting

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IRDye® conjugated secondary antibodies

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Multiplexing in fluorescent western blotting

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Secondary antibodies optimized for co-IP western blotting

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Can you provide the immunogen sequence?

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Benefits and Limitations of Primary and Secondary Antibody Conjugates

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Anti-mouse IgG for IP (HRP) (ab131368)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)

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Goat Anti-Human IgG Fc (HRP) (ab97225)

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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

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Goat Anti-Mouse IgG H&L (FITC) (ab6785)

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Alexa Fluor® 488-conjugated antibodies | Abcam

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