Secondary antibodies
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Secondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. They can be conjugated to fluorophores (eg Alexa Fluor® dyes), enzymes (eg HRP, alkaline phosphatase), or proteins (eg biotin) to amplify the signal of primary antibodies in a wide range of applications including western blot, immunohistochemistry, immunocytochemistry, ELISA, and flow cytometry.
Secondary antibodies are made by immunizing a host animal with antibodies from a different species. Immunizing a goat with rabbit IgG will generate goat anti-rabbit IgG secondary antibodies. The secondary antibody selected should match the species in which the primary antibody was raised in, as well as the isotype of the primary antibody of interest. For example, a goat anti-rabbit IgG secondary antibody can be used to detect the signal of rabbit IgG primary antibodies.
Read our secondary antibody selection guide to choose the right secondary for your experiment.
Find our most popular secondary antibodies by species:
Goat Anti-Rabbit IgG: Alexa Fluor®488 | Alexa Fluor®647 | Alexa Fluor®594 |HRP
Goat Anti-Mouse IgG: Alexa Fluor®488 | Alexa Fluor®647 |HRP
Donkey Anti-Rabbit IgG: Alexa Fluor®488 | Alexa Fluor®647
Donkey Anti-Mouse IgG: Alexa Fluor®488 | Alexa Fluor®647
Cross-adsorbed: Goat Anti-Mouse Alexa Fluor®488 | Goat Anti-Rabbit Alexa Fluor®647 | Donkey Anti-Rat Alexa Fluor®647
Features
Secondary antibody selection guide
Our guide will help you choose the most appropriate secondary antibody for your application.
IRDye® conjugated secondary antibodies
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Direct vs indirect IF
Understand the difference between direct and indirect methods for immunofluorescence.
Alexa Fluor® secondary antibodies
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 conjugated antibodies
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
HRP polymer secondaries
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
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ICC/IF protocol
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Immunoprecipitation protocol
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Recombinant antibodies: infinitely better
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IHC-validated antibodies for GeoMx® DSP Protein Barcoding
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Benefits and Limitations of Primary and Secondary Antibody Conjugates
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Mounting media and sera for imaging
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What is a clone number?
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Direct vs indirect immunofluorescence
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Indirect flow cytometry protocol
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Detect low-abundance proteins with biotinylated antibodies
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Gold conjugation kits
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Thoroughly validated directly conjugated antibodies
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Time-resolved fluorescence
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Understanding fluorophores
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Fluorochrome chart – a complete guide
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Fluorescence guide
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Non-antibody and small molecule labeling guide
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Antibody–oligonucleotide (Ab–oligo) conjugation| Abcam
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Antibody conjugation guide
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IHC/ICC Staining Techniques Using Single and Multiple Labels
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DyLight® Fluorochrome conjugated secondary antibodies
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Alexa Fluor® conjugated secondaries ideal for multicolor analysis
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HRP Secondary Antibodies
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Our guide to secondary antibody selection
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Hints and tips for fluorescent western blotting
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Multiplexing in fluorescent western blotting
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Secondary antibodies optimized for co-IP western blotting
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Conjugate selection guide for secondary antibodies
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How we validate our antibodies
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Alexa Fluor® 488-conjugated antibodies | Abcam
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Validated Alexa Fluor secondary antibodies for guaranteed performance
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A guide to fluorochromes poster
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Advanced Immunoprecipitation
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Principles and Practice of Confocal Microscopy in Life Sciences
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Double and triple immunostaining tips.
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Why choosing the right secondary antibody matters?
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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
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Alexa Fluor conjugated secondary antibodies - FAQs
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Flow cytometric analysis of extracellular vesicles
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Procedure for detection of phosphorylated proteins in western blot
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Secondaries you can trust
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Learn about cell imaging techniques through our protocol and webinar series
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IRDye® conjugated secondary antibodies
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Anti-mouse IgG for IP (HRP) (ab131368)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
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Goat Anti-Human IgG Fc (HRP) (ab97225)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
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Goat Anti-Mouse IgG H&L (FITC) (ab6785)
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