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Secondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. They can be conjugated to fluorophores (eg Alexa Fluor® dyes), enzymes (eg HRP, alkaline phosphatase), or proteins (eg biotin) to amplify the signal of primary antibodies in a wide range of applications including western blot, immunohistochemistry, immunocytochemistry, ELISA, and flow cytometry.
Secondary antibodies are made by immunizing a host animal with antibodies from a different species. Immunizing a goat with rabbit IgG will generate goat anti-rabbit IgG secondary antibodies. The secondary antibody selected should match the species in which the primary antibody was raised in, as well as the isotype of the primary antibody of interest. For example, a goat anti-rabbit IgG secondary antibody can be used to detect the signal of rabbit IgG primary antibodies.
Read our secondary antibody selection guide to choose the right secondary for your experiment.
Find our most popular secondary antibodies by species:
Goat Anti-Rabbit IgG: Alexa Fluor®488 | Alexa Fluor®647 | Alexa Fluor®594 |HRP|Gold 10nm|Gold 20nm
Goat Anti-Mouse IgG: Alexa Fluor®488 | Alexa Fluor®647 |HRP|Gold 10nm|Gold 20nm
Donkey Anti-Rabbit IgG: Alexa Fluor®488 | Alexa Fluor®647
Donkey Anti-Mouse IgG: Alexa Fluor®488 | Alexa Fluor®647
Cross-adsorbed: Goat Anti-Mouse Alexa Fluor®488 | Goat Anti-Rabbit Alexa Fluor®647 | Donkey Anti-Rat Alexa Fluor®647
Our guide will help you choose the most appropriate secondary antibody for your application.
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Understand the difference between direct and indirect methods for immunofluorescence.
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
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