Secondary antibodies
Product Categories
Over 1800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.
Features
Secondary antibody selection guide
Our guide will help you choose the most appropriate secondary antibody for your application.
IRDye® conjugated secondary antibodies
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Direct vs indirect IF
Understand the difference between direct and indirect methods for immunofluorescence.
Alexa Fluor® secondary antibodies
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 conjugated antibodies
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
HRP polymer secondaries
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
- All
- Articles
- Products
- Protocols
- Video
Indirect flow cytometry protocol
637267801000000000
Immunohistochemistry (IHC): the complete guide
637251095060000000
IRDye® conjugated secondary antibodies
637183179430000000
Learn about cell imaging techniques through our protocol and webinar series
637176369380000000
Alexa Fluor® conjugated secondaries ideal for multicolor analysis
637176359090000000
Conjugate selection guide for secondary antibodies
637048543170000000
Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
637048541450000000
Secondaries you can trust
636985267580000000
Double and triple immunostaining tips.
636984321280000000
Fluorochrome chart – a complete guide
636946504780000000
Anti-mouse IgG for IP (HRP) (ab131368)
636900555070000000
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
636899019720000000
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
636899017980000000
Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
636891035930000000
Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
636891035640000000
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
636891035390000000
Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
636891035180000000
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
636891034950000000
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
636891034660000000
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
636891034230000000
Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
636891033760000000
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
636891033480000000
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
636891033130000000
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
636891032870000000
Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
636888533820000000
Goat Anti-Human IgG Fc (HRP) (ab97225)
636888517320000000
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
636888514210000000
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
636888513360000000
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
636888456720000000
Our guide to secondary antibody selection
636870490820000000
Validated Alexa Fluor secondary antibodies for guaranteed performance
636868920610000000
ICC/IF protocol
636862488440000000
Goat Anti-Mouse IgG H&L (FITC) (ab6785)
636807232520000000
Secondary antibodies optimized for co-IP western blotting
636761571790000000
Hints and tips for fluorescent western blotting
636711372340000000
Direct vs indirect immunofluorescence
636565459360000000
Benefits and Limitations of Primary and Secondary Antibody Conjugates
636462641670000000
Why choosing the right secondary antibody matters?
636423627050000000
Multiplexing in fluorescent western blotting
636384735370000000
Alexa Fluor® 488-conjugated antibodies | Abcam
636354713140000000