Secondary antibodies
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Secondary antibodies are antibodies that bind to the protein of interest on a primary antibody in the indirect detection process. Secondaries are usually conjugated to fluorophores (eg Alexa Fluor® dyes), enzymes (eg HRP, alkaline phosphatase), or proteins (eg biotin). They are produced by immunizing a host animal with antibodies from a different species. For example, immunizing a goat with rabbit IgG would generate goat anti-rabbit IgG secondary antibodies.
When selecting a secondary antibody for your experiment, it should match the species the primary antibody was raised in and the isotype of the primary antibody of interest. For instance, the goat anti-rabbit IgG secondary antibody mentioned above can be used to detect the signal of a rabbit IgG primary antibody. Our secondary antibody selection guide can help you choose the right secondary antibody for your experiment.
Find our most popular secondary antibodies by species:
Goat Anti-Rabbit IgG: AF®488 | AF®647 | AF®594 |HRP
Goat Anti-Mouse IgG: AF®488 | AF®647 |HRP
Donkey Anti-Rabbit IgG: AF®488 | AF®647
Donkey Anti-Mouse IgG: AF®488 | AF®647
Cross-adsorbed: Goat Anti-Mouse AF®488 | Goat Anti-Rabbit AF®647 | Donkey Anti-Rat AF®647
Features
Secondary antibody selection guide
Our guide will help you choose the most appropriate secondary antibody for your application.
IRDye® conjugated secondary antibodies
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Direct vs indirect IF
Understand the difference between direct and indirect methods for immunofluorescence.
Alexa Fluor® secondary antibodies
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 conjugated antibodies
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
HRP polymer secondaries
Learn how to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC).
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Thoroughly validated directly conjugated antibodies
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Immunoprecipitation protocol
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Hints and tips for fluorescent western blotting
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Our guide to secondary antibody selection
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Conjugate selection guide for secondary antibodies
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How we validate our antibodies
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Mounting media and sera for imaging
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Alexa Fluor® 488-conjugated antibodies | Abcam
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Validated Alexa Fluor secondary antibodies for guaranteed performance
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Secondary antibodies optimized for co-IP western blotting
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Indirect flow cytometry protocol
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A guide to fluorochromes poster
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Fluorochrome chart – a complete guide
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Direct vs indirect immunofluorescence
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Understanding fluorophores
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Advanced Immunoprecipitation
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Principles and Practice of Confocal Microscopy in Life Sciences
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Double and triple immunostaining tips.
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ICC/IF protocol
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Immunohistochemistry (IHC): the complete guide
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Why choosing the right secondary antibody matters?
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Alexa Fluor® conjugated secondaries ideal for multicolor analysis
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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
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Alexa Fluor conjugated secondary antibodies - FAQs
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Fluorescent western blot Alexa Fluor® secondaries
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Flow cytometric analysis of extracellular vesicles
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Detect low-abundance proteins with biotinylated antibodies
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Procedure for detection of phosphorylated proteins in western blot
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Secondaries you can trust
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Learn about cell imaging techniques through our protocol and webinar series
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IRDye® conjugated secondary antibodies
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Multiplexing in fluorescent western blotting
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Benefits and Limitations of Primary and Secondary Antibody Conjugates
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Anti-mouse IgG for IP (HRP) (ab131368)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
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Goat Anti-Human IgG Fc (HRP) (ab97225)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
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Goat Anti-Mouse IgG H&L (FITC) (ab6785)
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