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Home Secondary antibodies

Alexa Fluor® conjugated secondaries

Product
  • Alexa Fluor® 405
  • Alexa Fluor® 488
  • Alexa Fluor® 555
  • Alexa Fluor® 568
  • Alexa Fluor® 594
  • Alexa Fluor® 647
  • Alexa Fluor® 680
  • Alexa Fluor® 750
  • Alexa Fluor® 790

Alexa Fluor® are the brightest and most trusted fluorescent dyes. Our manufacturing processes ensure Alexa Fluor® conjugated secondary antibodies of high specificity and sensitivity. We offer secondary antibodies conjugated to nine Alexa Fluor® dyes that cover the whole spectrum with minimal spectral overlap, thus ideal for multi-color analysis.

Browse all related products

Features

Alexa Fluor® secondaries

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Double and triple immunostaining tips

These simple tips will minimize background staining in your experiments as well as prevent false positives or negatives.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.


Fluorochrome chart

A quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

Alexa Fluor® secondaries FAQs

View the answers to commonly asked questions about Alexa Fluor® secondaries usage.


Alexa Fluor® 488 conjugated antibodies

Alexa Fluor® 488  has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.

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  • Articles
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  • Protocol

ICC/IF protocol

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Protocol

Direct vs indirect immunofluorescence

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Article

nti-BRG1 antibody IF staining

Alexa Fluor® conjugated secondaries ideal for multicolor analysis

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Article

A guide to fluorochromes poster

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Pathway

Fluorochrome chart – a complete guide

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Article

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083)

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Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

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Product

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Product

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)

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Product

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

636888461030000000

Product

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)

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Product

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (ab150131)

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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

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Understanding secondary antibodies

Our guide to secondary antibody selection

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Why choosing the right secondary antibody matters?

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Alexa Fluor® 488-conjugated antibodies | Abcam

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