Home Secondary antibodies

Alexa Fluor® conjugated secondaries

Alexa Fluor® are the brightest and most trusted fluorescent dyes. Our manufacturing processes ensure Alexa Fluor® conjugated secondary antibodies of high specificity and sensitivity. We offer secondary antibodies conjugated to nine Alexa Fluor® dyes that cover the whole spectrum with minimal spectral overlap, thus ideal for multi-color analysis.


Alexa Fluor® secondaries

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Double and triple immunostaining tips

These simple tips will minimize background staining in your experiments as well as prevent false positives or negatives.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.

Fluorochrome chart

A quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

Alexa Fluor® secondaries FAQs

View the answers to commonly asked questions about Alexa Fluor® secondaries usage.

Alexa Fluor® 488 conjugated antibodies

Alexa Fluor® 488  has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.

  • All
  • Articles
  • Pathway
  • Products
  • Protocol
Understanding secondary antibodies

ICC/IF protocol


A guide to fluorochromes poster


Sign up