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Home Secondary antibodies

Pre-adsorbed antibodies

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Pre-adsorbed (cross-adsorbed) secondary antibodies have gone through an extra purification step to eliminate antibodies that may cross-react with other species, minimizing non-specific binding and high background. We offer more than 1,000 pre-adsorbed secondaries conjugated to diverse molecules including HRP and Alexa Fluor® dyes.

Browse all related products

Features

Pre-adsorbed secondaries

Learn how you can minimize non-specific binding and high background staining.

Alexa Fluor® secondaries

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Secondary antibody selection guide

Our guide to help you choose the most appropriate secondary antibody for your experiment.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.

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ICC/IF protocol

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Protocol

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IHC/ICC Staining Techniques Using Single and Multiple Labels

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Video

Direct vs indirect immunofluorescence

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Article

nti-BRG1 antibody IF staining

Alexa Fluor® conjugated secondaries ideal for multicolor analysis

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Article

Fluorochrome chart – a complete guide

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Article

Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165)

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Product

Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

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Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133)

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Product

Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)

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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175649)

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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173)

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

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Product

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175670)

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Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150084)

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Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175475)

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Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

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Product

Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

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Product

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)

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Product

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083)

636899018430000000

Product

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)

636899018190000000

Product

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

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Product

Understanding secondary antibodies

Our guide to secondary antibody selection

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Article

Pre-adsorbed secondary antibodies - minimize non-specific binding and high background staining

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Article

Why choosing the right secondary antibody matters?

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Article

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