Home Secondary antibodies

Pre-adsorbed antibodies

Pre-adsorbed (cross-adsorbed) secondary antibodies have gone through an extra purification step to eliminate antibodies that may cross-react with other species, minimizing non-specific binding and high background. We offer more than 1,000 pre-adsorbed secondaries conjugated to diverse molecules including HRP and Alexa Fluor® dyes.


Pre-adsorbed secondaries

Learn how you can minimize non-specific binding and high background staining.

Alexa Fluor® secondaries

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Secondary antibody selection guide

Our guide to help you choose the most appropriate secondary antibody for your experiment.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.

  • All
  • Articles
  • Products
  • Protocol
  • Video
Understanding secondary antibodies

ICC/IF protocol


Sign up