Product nameAnti-Nav1.6/SCN8A antibody
See all Nav1.6/SCN8A primary antibodies
DescriptionRabbit polyclonal to Nav1.6/SCN8A
Tested applicationsSuitable for: IHC-FrFl, ICC/IF, IHC-P, IHC-Fr, WB, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Nav1.6/SCN8A.
This product was previously labelled as Nav1.6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab65166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration. See Abreview.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 220 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 27905510|
FunctionMediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.
Tissue specificityIsoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsMay be ubiquitinated by NEDD4L; which would promote its endocytosis.
Cellular localizationMembrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
- Information by UniProt
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PFA perfusion-fixed sections of rat hippocampus (following 21 fdays of KA treatment) using ab65166 at 1/600 dilution (red). Co-localization with Nestin (green) is shown.
Data taken from PMID 27905510 (Figure 7E).
Remarkable alterations of Nav1.6/SCN8A in reactive astrogliosis during epileptogenesis
HongyanZhu, Yuxiao Zhao*, HaoWu, Nan Jiang, ZiyWang, Weide Lin, Jiahui Jin & Yonghua Ji
Scientific Reports | 6:38108 | DOI: 10.1038/srep38108
This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
This product has been referenced in:
- Arechederra M et al. Hypermethylation of gene body CpG islands predicts high dosage of functional oncogenes in liver cancer. Nat Commun 9:3164 (2018). Read more (PubMed: 30089774) »
- Zhu H et al. Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis. Sci Rep 6:38108 (2016). IHC-P ; Rat . Read more (PubMed: 27905510) »