For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for calling and bringing this issue to our attention.
I just wanted to make sure you were loading the correct amount of protein and to make another suggestion regarding the blocking step.
For the brain lysate, ab30151, you will want to load at least 10ug, which is a volume of about 4 microliters.
For the blocking step, you may find that a relatively short block (30 minutes) instead of overnight, followed by overnight incubation with the antibody will help develop the signal, compared to an overnight block followed by a shorter incubation with the primary. These are little modifications, though. I suspect that either the lysate is defective or the transfer was not optimal. Please let me know how your tests go. If they fail, we will be happy to discuss a replacement of either the antibody or the lysate, or both.
Answered on May 07 2012