For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Inquiry: Dear Sir/Madam, I recently bought an anti-Nav 1.6 antibody from abcam. However, it does not appear to be effective. I have used it to stain longitudinal frozen sections of spinal cord tissue (fixed using PFA) and the purpose of staining is to identify the node of Ranvier. Other antibodies have successfully identified the juxtaparanodal region and paranode. However, in sections co-stained with the sodium channel antibody, the node does not fluoresce. I have used a concentration of up to 1:100, tried with and without antigen retrieval and incubated the sections for up to 24 hours at room temperature. I should be grateful for any advice that you may be able to offer. Yours sincerely,
Asked on Aug 07 2012
I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.
As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.
Could you please provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.
Answered on Aug 07 2012