Overview

  • Product name

    Anti-Nav1.6/SCN8A antibody - Membrane Vesicle Marker
    See all Nav1.6/SCN8A primary antibodies
  • Description

    Rabbit polyclonal to Nav1.6/SCN8A - Membrane Vesicle Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Monkey
  • Immunogen

    Synthetic peptide within Human Nav1.6/SCN8A (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9UQD0

  • Positive control

    • WB: HEK-293T, RAW 264.7 and PC-12 whole cell lysate. IHC-P: Human skeletal muscle and cortex tissue.
  • General notes

     This product was previously labelled as Nav1.6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 225 kDa.
IHC-P 1/100.

Target

  • Function

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.
  • Tissue specificity

    Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    May be ubiquitinated by NEDD4L; which would promote its endocytosis.
  • Cellular localization

    Membrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CerIII antibody
    • CIAT antibody
    • EIEE13 antibody
    • hNa6/Scn8a voltage gated sodium channel antibody
    • MED antibody
    • Motor endplate disease antibody
    • NaCh 6 antibody
    • NaCh6 antibody
    • Nav 1.6 antibody
    • Nbna1 antibody
    • peripheral nerve protein type 4 antibody
    • PN 4 antibody
    • PN4 antibody
    • SCN8A antibody
    • SCN8A_HUMAN antibody
    • Sodium channel protein type 8 alpha subunit antibody
    • Sodium channel protein type 8 subunit alpha antibody
    • Sodium channel protein type VIII alpha subunit antibody
    • Sodium channel protein type VIII subunit alpha antibody
    • Sodium channel voltage gated type VIII alpha antibody
    • Sodium channel voltage gated type VIII alpha polypeptide antibody
    • Sodium channel voltage gated type VIII alpha subunit antibody
    • Voltage gated sodium channel subunit alpha Nav1.6 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.6 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Nav1.6/SCN8A at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cortex tissue stained for Nav1.6/SCN8A at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-Nav1.6/SCN8A antibody - Membrane Vesicle Marker (ab230654) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 225 kDa

References

ab230654 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230654.
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