Product nameAnti-Nav1.7 antibody
See all Nav1.7 primary antibodies
DescriptionRabbit polyclonal to Nav1.7
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Nav1.7 aa 1000-1100. The exact sequence is proprietary.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab65167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 226 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21441906|
FunctionMediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784).
Tissue specificityExpressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
Involvement in diseasePrimary erythermalgia
Indifference to pain, congenital, autosomal recessive
Paroxysmal extreme pain disorder
Generalized epilepsy with febrile seizures plus 7
Febrile seizures, familial, 3B
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsPhosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.
Cellular localizationCell membrane. Cell projection. In neurite terminals.
- Information by UniProt
- ETHA antibody
- GEFSP7 antibody
- hNE Na antibody
ab65167 staining Nav1.7 in human sperm cells by Immunocytochemistry/ Immunofluorescence.
Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab65167 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.
ICC/IF image of ab65167 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65167, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat dorsal root ganglia, staining Nav1.7 with ab65167.
Tissue was blocked with 1% BSA and permeabilized with 0.01% Triton X-100, before incubating with primary antibody (1/500 in PBS with 0.1% BSA + 0.01% Triton X-100) was overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.
This product has been referenced in:
- Henningsen K et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22311638) »
- Weiss J et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21441906) »