Overview

  • Product name

  • Description

    Rabbit polyclonal to Nav1.7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Nav1.7 aa 1000-1100. The exact sequence is proprietary.
    (Peptide available as ab192016)

Properties

Applications

Our Abpromise guarantee covers the use of ab65167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 226 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21441906

Target

  • Function

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784).
  • Tissue specificity

    Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
  • Involvement in disease

    Primary erythermalgia
    Indifference to pain, congenital, autosomal recessive
    Paroxysmal extreme pain disorder
    Generalized epilepsy with febrile seizures plus 7
    Febrile seizures, familial, 3B
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
    Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.
  • Cellular localization

    Cell membrane. Cell projection. In neurite terminals.
  • Information by UniProt
  • Database links

  • Alternative names

    • ETHA antibody
    • GEFSP7 antibody
    • hNE Na antibody
    • hNE-Na antibody
    • hNENa antibody
    • NE NA antibody
    • NENA antibody
    • Neuroendocrine sodium channel antibody
    • Peripheral sodium channel 1 antibody
    • PN1 antibody
    • Scn9a antibody
    • SCN9A_HUMAN antibody
    • Sodium channel protein type 9 subunit alpha antibody
    • Sodium channel protein type IX subunit alpha antibody
    • Sodium channel voltage gated type IX alpha antibody
    • Sodium channel voltage gated type IX alpha polypeptide antibody
    • Sodium channel voltage gated type IX alpha subunit antibody
    • Voltage gated sodium channel alpha subunit Nav1.7 antibody
    • Voltage gated sodium channel subunit alpha Nav1 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.7 antibody
    see all

Images

  • ab65167 staining Nav1.7 in human sperm cells by Immunocytochemistry/ Immunofluorescence.
    Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab65167 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.
  • ICC/IF image of ab65167 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65167, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat dorsal root ganglia, staining Nav1.7 with ab65167.

    Tissue was blocked with 1% BSA and permeabilized with 0.01% Triton X-100, before incubating with primary antibody (1/500 in PBS with 0.1% BSA + 0.01% Triton X-100) was overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.

References

This product has been referenced in:

  • Henningsen K  et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22311638) »
  • Weiss J  et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21441906) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 14 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (DRG / Brain)
Loading amount
10 µg
Specification
DRG / Brain
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 24 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification
Dorsal Root Ganglia (DRG) neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - tween 20
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Answer

Thank you for contacting us.

Your credit note ID is xx


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

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The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

Thank you for taking time to put the information together and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

As a free of charge replacement we can offer you another vial of this same antibody or an alternative antibody. The new vial would obviously also be covered by our Abpromise guarantee. We have currently only the same batch of ab65167in stock. However we have not received any other negative feedback on this batch or on any batch during the last two years.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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