Anti-Nav1.7 antibody [N68/6] (ab85015)
Key features and details
- Mouse monoclonal [N68/6] to Nav1.7
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Nav1.7 antibody [N68/6]
See all Nav1.7 primary antibodies -
Description
Mouse monoclonal [N68/6] to Nav1.7 -
Host species
Mouse -
Specificity
Some customers have used this antibody successfully in rat, however our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
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Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse dorsal root ganglion tissue; Mouse back skin tissue.
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General notes
The clone number has been updated from S68-6 to N68/6, both clone numbers name the same antibody clone.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N68/6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784). -
Tissue specificity
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion. -
Involvement in disease
Primary erythermalgia
Indifference to pain, congenital, autosomal recessive
Paroxysmal extreme pain disorder
Generalized epilepsy with febrile seizures plus 7
Febrile seizures, familial, 3B -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4. -
Cellular localization
Cell membrane. Cell projection. In neurite terminals. - Information by UniProt
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Database links
- Entrez Gene: 6335 Human
- Entrez Gene: 20274 Mouse
- Entrez Gene: 78956 Rat
- Omim: 603415 Human
- SwissProt: Q15858 Human
- SwissProt: Q62205 Mouse
- SwissProt: O08562 Rat
- Unigene: 439145 Human
see all -
Alternative names
- ETHA antibody
- GEFSP7 antibody
- hNE Na antibody
see all
Images
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IHC image of Nav1.7 staining in mouse dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85015, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab85015 has been referenced in 9 publications.
- Gonzales J et al. Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers. Mol Pharm 18:940-951 (2021). PubMed: 33404254
- Tian JJ et al. Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain. Int J Mol Med 46:782-794 (2020). PubMed: 32468069
- Jia Q et al. Activating Sirt1 by resveratrol suppresses Nav1.7 expression in DRG through miR-182 and alleviates neuropathic pain in rats. Channels (Austin) 14:69-78 (2020). PubMed: 32089065
- Gonzales J et al. Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization. EJNMMI Res 10:49 (2020). PubMed: 32409881
- Gonzales J et al. Fluorescence Imaging of Peripheral Nerves by a Nav1.7-Targeted Inhibitor Cystine Knot Peptide. Bioconjug Chem 30:2879-2888 (2019). PubMed: 31647222
- Xie MX et al. Nuclear Factor-kappaB Gates Nav1.7 Channels in DRG Neurons via Protein-Protein Interaction. iScience 19:623-633 (2019). PubMed: 31446225
- Hofmann L et al. Characterization of small fiber pathology in a mouse model of Fabry disease. Elife 7:N/A (2018). PubMed: 30328411
- Bi RY et al. Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ. PLoS One 12:e0178589 (2017). IHC-Fr ; Rat . PubMed: 28582470
- Bi RY et al. Involvement of trigeminal ganglionic Nav 1.7 in hyperalgesia of inflamed temporomandibular joint is dependent on ERK1/2 phosphorylation of glial cells in rats. Eur J Pain 17:983-94 (2013). PubMed: 23242737