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    nav18scn10a-antibody-ab139510.pdf

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Neuroscience Neurotransmission Receptors / Channels Sodium Channels
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Anti-Nav1.8/SCN10A antibody (ab139510)

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Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)

    Key features and details

    • Rabbit polyclonal to Nav1.8/SCN10A
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Nav1.8/SCN10A antibody
      See all Nav1.8/SCN10A primary antibodies
    • Description

      Rabbit polyclonal to Nav1.8/SCN10A
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse Nav1.8/SCN10A aa 1850-1950 conjugated to keyhole limpet haemocyanin.
      Database link: Q6QIY3

    • Positive control

      • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat Cerebellum.
    • General notes

       This product was previously labelled as Nav1.8

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • Sodium Channels
      • Neuroscience
      • Sensory System
      • Somatosensory system
      • Nociception

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab139510 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Rat
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).

    Target

    • Function

      Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
    • Tissue specificity

      Expressed in the dorsal root ganglia and sciatic nerve.
    • Involvement in disease

      Episodic pain syndrome, familial, 2
    • Sequence similarities

      Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
      Contains 1 IQ domain.
    • Domain

      The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
    • Post-translational
      modifications

      Ubiquitinated by NEDD4L; which promotes its endocytosis.
      Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
    • Cellular localization

      Cell membrane. It can be translocated to the cell membrane through association with S100A10.
    • Target information above from: UniProt accession Q9Y5Y9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 20264 Mouse
      • Entrez Gene: 29571 Rat
      • SwissProt: P70276 Mouse
      • SwissProt: Q6QIY3 Mouse
      • SwissProt: Q62968 Rat
      • Unigene: 247042 Mouse
      • Unigene: 455125 Mouse
      • Unigene: 489505 Mouse
      • Unigene: 10246 Rat
      see all
    • Alternative names

      • hPN3 antibody
      • mPN3 antibody
      • Peripheral nerve sodium channel 3 antibody
      • Pn3 (gene name) antibody
      • PN3 antibody
      • SCN10A antibody
      • SCNAA_HUMAN antibody
      • Sensory neuron sodium channel antibody
      • Sns (gene name) antibody
      • SNS antibody
      • Sodium channel protein type 10 subunit alpha antibody
      • Sodium channel protein type X alpha subunit antibody
      • Sodium channel protein type X subunit alpha antibody
      • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
      • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
      see all

    Images

    • Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)
      Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)
      All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Cerebellum Mouse Tissue Lysate
      Lane 3 : Cerebellum Rat Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 221 kDa
      Observed band size: 260 kDa
      why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      Nav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab139510? Please let us know so that we can cite the reference in this datasheet.

    ab139510 has not yet been referenced specifically in any publications.

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