Product nameAnti-Nav1.8/SCN10A antibody
See all Nav1.8/SCN10A primary antibodies
DescriptionRabbit polyclonal to Nav1.8/SCN10A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide corresponding to Mouse Nav1.8/SCN10A aa 1850-1950 conjugated to keyhole limpet haemocyanin.
Database link: Q6QIY3
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat Cerebellum.
This product was previously labelled as Nav1.8
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).|
FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
Involvement in diseaseEpisodic pain syndrome, familial, 2
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
- Information by UniProt
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 221 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Nav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab139510 has not yet been referenced specifically in any publications.