Overview

  • Product name

    Anti-Nav1.8/SCN10A antibody
    See all Nav1.8/SCN10A primary antibodies
  • Description

    Rabbit polyclonal to Nav1.8/SCN10A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Nav1.8/SCN10A aa 1850-1950 conjugated to keyhole limpet haemocyanin.
    Database link: Q6QIY3

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat Cerebellum.
  • General notes

     This product was previously labelled as Nav1.8

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).

Target

  • Function

    Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
  • Tissue specificity

    Expressed in the dorsal root ganglia and sciatic nerve.
  • Involvement in disease

    Episodic pain syndrome, familial, 2
  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.
  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • Cellular localization

    Cell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

Images

  • All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 221 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Nav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab139510 has not yet been referenced specifically in any publications.

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