Anti-Nav1.8/SCN10A antibody (ab139510)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Nav1.8/SCN10A antibody
See all Nav1.8/SCN10A primary antibodies -
Description
Rabbit polyclonal to Nav1.8/SCN10A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Nav1.8/SCN10A aa 1850-1950 conjugated to keyhole limpet haemocyanin.
Database link: Q6QIY3 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat Cerebellum.
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General notes
This product was previously labelled as Nav1.8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab139510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa). |
Target
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Function
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
Tissue specificity
Expressed in the dorsal root ganglia and sciatic nerve. -
Involvement in disease
Episodic pain syndrome, familial, 2 -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
Cellular localization
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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Database links
- Entrez Gene: 20264 Mouse
- Entrez Gene: 29571 Rat
- SwissProt: P70276 Mouse
- SwissProt: Q6QIY3 Mouse
- SwissProt: Q62968 Rat
- Unigene: 247042 Mouse
- Unigene: 455125 Mouse
- Unigene: 489505 Mouse
see all -
Alternative names
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
Images
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Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 221 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesNav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab139510 has not yet been referenced specifically in any publications.
