Key features and details
- Rabbit polyclonal to Nav1.8/SCN10A
- Suitable for: WB, IHC-Fr
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Nav1.8/SCN10A antibody
See all Nav1.8/SCN10A primary antibodies
DescriptionRabbit polyclonal to Nav1.8/SCN10A
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Rat
Synthetic peptide corresponding to Nav1.8/SCN10A aa 1892-1956.
This product was previously labelled as Nav1.8
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituents: PBS, Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab63331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/4000. Predicted molecular weight: 217 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
Involvement in diseaseEpisodic pain syndrome, familial, 2
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
- Information by UniProt
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
Western blot analysis. A single band of strong NaV1.8/SCN10A-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8/SCN10A cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.
Immunohistochemistry was performed on 2 week old rat lumber DRG sections. NaV1.8/SCN10A-like immunoreactivity can be detected only in small diameter (<30 um) neurons.
ab63331 has been referenced in 2 publications.
- Desiderio S et al. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Rep 26:3522-3536.e5 (2019). PubMed: 30917309
- Isensee J et al. Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216-29 (2014). PubMed: 24190886