Anti-Nav1.8/SCN10A antibody (ab63331)
Key features and details
- Rabbit polyclonal to Nav1.8/SCN10A
- Suitable for: ICC, WB, IHC-Fr
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Nav1.8/SCN10A antibody
See all Nav1.8/SCN10A primary antibodies -
Description
Rabbit polyclonal to Nav1.8/SCN10A -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Frmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Nav1.8/SCN10A aa 1892-1956.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
1/4000. Predicted molecular weight: 217 kDa.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/4000. Predicted molecular weight: 217 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
Tissue specificity
Expressed in the dorsal root ganglia and sciatic nerve. -
Involvement in disease
Episodic pain syndrome, familial, 2 -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
Cellular localization
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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Database links
- Entrez Gene: 29571 Rat
- SwissProt: Q62968 Rat
- Unigene: 10246 Rat
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Alternative names
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
Images
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Western blot analysis. A single band of strong NaV1.8/SCN10A-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8/SCN10A cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.
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Immunohistochemistry was performed on 2 week old rat lumber DRG sections. NaV1.8/SCN10A-like immunoreactivity can be detected only in small diameter (<30 um) neurons.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab63331 has been referenced in 2 publications.
- Desiderio S et al. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Rep 26:3522-3536.e5 (2019). PubMed: 30917309
- Isensee J et al. Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216-29 (2014). PubMed: 24190886