Key features and details
- Mouse monoclonal [N134/12] to Nav1.8/SCN10A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG2a
Product nameAnti-Nav1.8/SCN10A antibody [N134/12]
See all Nav1.8/SCN10A primary antibodies
DescriptionMouse monoclonal [N134/12] to Nav1.8/SCN10A
SpecificityNo cross reactivity against other Nav channels.
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Fusion protein corresponding to Rat Nav1.8/SCN10A aa 1724-1956.
Database link: Q62968
- WB: COS cell lysate transiently expressing Nav1.8/SCN10A. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue.
The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.
This product was previously labelled as Nav1.8
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
Involvement in diseaseEpisodic pain syndrome, familial, 2
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
- Information by UniProt
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
Paraffin-embedded mouse backskin tissue stained for Nav1.8/SCN10A using ab93616 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616) at 1/1000 dilution + Monkey COS cell lysate transiently expressing Nav1.8/SCN10A. at 15 µg
Sheep Anti-Mouse IgG: HRP
Predicted band size: 220 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93616 has been referenced in 5 publications.
- Ni S et al. Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice. Nat Commun 10:5643 (2019). PubMed: 31822662
- Goldstein RH et al. Location and Plasticity of the Sodium Spike Initiation Zone in Nociceptive Terminals In Vivo. Neuron 102:801-812.e5 (2019). PubMed: 30926280
- Cejudo-Roman A et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . PubMed: 24086692
- Ramachandra R et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). PubMed: 23843437
- Ramachandra R et al. Tetrodotoxin-resistant voltage-dependent sodium channels in identified muscle afferent neurons. J Neurophysiol 108:2230-41 (2012). IHC . PubMed: 22855776