Product nameAnti-Nav1.8/SCN10A antibody [N134/12]
See all Nav1.8/SCN10A primary antibodies
DescriptionMouse monoclonal [N134/12] to Nav1.8/SCN10A
SpecificityNo cross reactivity against other Nav channels.
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Fusion protein corresponding to Rat Nav1.8/SCN10A aa 1724-1956.
Database link: Q62968
- WB: COS cell lysate transiently expressing Nav1.8/SCN10A. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue.
The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.
This product was previously labelled as Nav1.8
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|ICC||Use a concentration of 0.1 - 1 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
Involvement in diseaseEpisodic pain syndrome, familial, 2
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
- Information by UniProt
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
Paraffin-embedded mouse backskin tissue stained for Nav1.8/SCN10A using ab93616 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616) at 1/1000 dilution + Monkey COS cell lysate transiently expressing Nav1.8/SCN10A. at 15 µg
Sheep Anti-Mouse IgG: HRP
Predicted band size: 220 kDa
This product has been referenced in:
- Cejudo-Roman A et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24086692) »
- Ramachandra R et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). Read more (PubMed: 23843437) »