Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Tissue specificityBroadly expressed, with highest levels in heart and skeletal muscle, and lowest levels in liver, small intestine and thymus. Up-regulated together with N-myc in some neuroblastoma cell lines.
Sequence similaritiesContains 2 WD repeats.
- Information by UniProt
- NBAS antibody
- NBAS_HUMAN antibody
- Neuroblastoma-amplified gene protein antibody
- Neuroblastoma-amplified sequence antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab122370 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122370, at a 1/50 dilution, staining NBAS in paraffin embedded Human heart muscle tissue by Immunohistochemistry.