Overview

  • Product name

  • Description

    Rabbit polyclonal to NBAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: AKGDLKFPLK IFQHSKPDLQ QKIIPDQDQL MAIALECIYT CERNDQLCLC YDLLECLPER GYGDKTEATT KLHDMVDQLE QILSVS, corresponding to amino acids 962-1047 of Human NBAS (UniProt ID: A2RRP1).

  • Positive control

    • Human heart muscle tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity

    Broadly expressed, with highest levels in heart and skeletal muscle, and lowest levels in liver, small intestine and thymus. Up-regulated together with N-myc in some neuroblastoma cell lines.
  • Sequence similarities

    Contains 2 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • NBAS antibody
    • NBAS_HUMAN antibody
    • Neuroblastoma-amplified gene protein antibody
    • Neuroblastoma-amplified sequence antibody

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab122370 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122370, at a 1/50 dilution, staining NBAS in paraffin embedded Human heart muscle tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Xu D  et al. Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions. J Cell Biol 217:975-995 (2018). Read more (PubMed: 29367353) »
See 1 Publication for this product

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