NBD-Cl, Amine-reactive fluorogenic dye (ab145315)
- Datasheet
- References
Overview
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Product name
NBD-Cl, Amine-reactive fluorogenic dye -
Description
Amine-reactive, fluorogenic dye -
Biological description
Amine-reactive, fluorogenic dye. Forms green fluorescent adducts with aliphatic amines and thiols. Labels proteins, peptides, amino acids and other biomolecules. -
Purity
> 98% -
CAS Number
10199-89-0 -
Chemical structure
Properties
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Excitation
337nm -
Emission
337nm -
Chemical name
4-Chloro-7-nitro-2,1,3-benzoxadiazole -
Molecular weight
199.55 -
Molecular formula
C6H2ClN3O3 -
PubChem identifier
25043 -
Storage instructions
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=C(C2=NON=C2C(=C1)Cl)[N+](=O)[O-] -
Source
Synthetic
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Research areas
References
This product has been referenced in:
- El-Kosasy AM et al. Screening and Optimization of the Reaction of Polymyxin B Sulphate with NBD-Cl for the Synchronous Spectrofluorimetric Determination of Polymyxin B Sulphate in Human Plasma. J Fluoresc 25:695-705 (2015). Read more (PubMed: 25773711) »
- Walash MI et al. Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4-chloro-7--nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Luminescence : (2015). Read more (PubMed: 25783013) »
- Shehata MA et al. Utilization of 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) for kinetic spectrophotometric assay of befunolol hydrochloride in its pharmaceutical formulation. J AOAC Int 89:646-50 (0). Read more (PubMed: 16792064) »