Overview

  • Product name

    NBD-Cl, Amine-reactive fluorogenic dye
  • Description

    Amine-reactive, fluorogenic dye
  • Biological description

    Amine-reactive, fluorogenic dye. Forms green fluorescent adducts with aliphatic amines and thiols. Labels proteins, peptides, amino acids and other biomolecules.
  • Purity

    > 98%
  • CAS Number

    10199-89-0
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    337nm
  • Emission

    337nm
  • Chemical name

    4-Chloro-7-nitro-2,1,3-benzoxadiazole
  • Molecular weight

    199.55
  • Molecular formula

    C6H2ClN3O3
  • PubChem identifier

    25043
  • Storage instructions

    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1=C(C2=NON=C2C(=C1)Cl)[N+](=O)[O-]
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • El-Kosasy AM  et al. Screening and Optimization of the Reaction of Polymyxin B Sulphate with NBD-Cl for the Synchronous Spectrofluorimetric Determination of Polymyxin B Sulphate in Human Plasma. J Fluoresc 25:695-705 (2015). Read more (PubMed: 25773711) »
  • Walash MI  et al. Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4-chloro-7--nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Luminescence : (2015). Read more (PubMed: 25783013) »
  • Shehata MA  et al. Utilization of 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) for kinetic spectrophotometric assay of befunolol hydrochloride in its pharmaceutical formulation. J AOAC Int 89:646-50 (0). Read more (PubMed: 16792064) »

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