Overview

  • Product name

    NBD-F, Pro-fluorescent reagent
  • Description

    Pro-fluorescent reagent which forms a fluorescent product on reaction with primary or secondary amines.
  • Alternative names

    • 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
    • 4-Fluoro-7-Nitrobenz-2-Oxa-1,3-Diazole
    • 4-Fluoro-7-nitrobenzofurazan
    • 7-Fluoro-4-nitrobenzo-2-oxa-1,3-diazole
    • NBD fluoride
    see all
  • Biological description

    Pro-fluorescent reagent which forms a fluorescent product on reaction with primary or secondary amines. Similar to NBD chloride (ab145315) except that it is much more reactive toward amines. Both reagents are nonfluorescent until they react with primary or secondary amines to produce a fluorescent product. Extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

  • Purity

    > 98%
  • CAS Number

    29270-56-2
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    465nm
  • Emission

    535nm
  • Chemical name

    4-Fluoro-7-nitrobenzofurazan
  • Molecular weight

    183.10
  • Molecular formula

    C6H2FN3O3
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Miyamoto T  et al. Origin of D-amino acids detected in the acid hydrolysates of purified Escherichia coli ß-galactosidase. J Pharm Biomed Anal 116:105-8 (2015). Read more (PubMed: 25999172) »
  • Suzuki S  et al. Rapid analysis of amino sugars by microchip electrophoresis with laser-induced fluorescence detection. Electrophoresis 22:4023-31 (2001). Read more (PubMed: 11700736) »
  • Tani M  et al. Enzymatic synthesis of omega-amino-ceramide: preparation of a sensitive fluorescent substrate for ceramidase. Anal Biochem 263:183-8 (1998). Read more (PubMed: 9799530) »
  • Watanabe Y & Imai K High-performance liquid chromatography and sensitive detection of amino acids derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole. Anal Biochem 116:471-2 (1981). Read more (PubMed: 7316179) »

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