Product nameNBD-F, Pro-fluorescent reagent
DescriptionPro-fluorescent reagent which forms a fluorescent product on reaction with primary or secondary amines.
Pro-fluorescent reagent which forms a fluorescent product on reaction with primary or secondary amines. Similar to NBD chloride (ab145315) except that it is much more reactive toward amines. Both reagents are nonfluorescent until they react with primary or secondary amines to produce a fluorescent product. Extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Miyamoto T et al. Origin of D-amino acids detected in the acid hydrolysates of purified Escherichia coli ß-galactosidase. J Pharm Biomed Anal 116:105-8 (2015). Read more (PubMed: 25999172) »
- Suzuki S et al. Rapid analysis of amino sugars by microchip electrophoresis with laser-induced fluorescence detection. Electrophoresis 22:4023-31 (2001). Read more (PubMed: 11700736) »
- Tani M et al. Enzymatic synthesis of omega-amino-ceramide: preparation of a sensitive fluorescent substrate for ceramidase. Anal Biochem 263:183-8 (1998). Read more (PubMed: 9799530) »
- Watanabe Y & Imai K High-performance liquid chromatography and sensitive detection of amino acids derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole. Anal Biochem 116:471-2 (1981). Read more (PubMed: 7316179) »