Recombinant
RabMAb

Recombinant Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)

Overview

  • Product name

    Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal
    See all NBEAL2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14501(B)] to NBEAL2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NBEAL2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q6ZNJ1

  • Positive control

    • Human platelet lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 303 kDa (predicted molecular weight: 303 kDa).

Target

  • Function

    Probably involved in thrombopoiesis. Plays a role in the development or secretion of alpha-granules, that contain several growth factors important for platelet biogenesis.
  • Tissue specificity

    Expressed in megakaryocytes.
  • Involvement in disease

    Gray platelet syndrome
  • Sequence similarities

    Belongs to the WD repeat neurobeachin family.
    Contains 1 BEACH domain.
    Contains 5 WD repeats.
  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDPLT4 antibody
    • GPS antibody
    • NBEAL2 antibody
    • NBEL2_HUMAN antibody
    • Neurobeachin-like protein 2 antibody
    • UNQ253/PRO290 antibody
    see all

Images

  • Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162) at 1/1000 dilution + Human platelet lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 303 kDa

References

This product has been referenced in:

  • Tomberg K  et al. Spontaneous 8bp Deletion in Nbeal2 Recapitulates the Gray Platelet Syndrome in Mice. PLoS One 11:e0150852 (2016). WB . Read more (PubMed: 26950939) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (peripheral blood cells)
Permeabilization
Yes - eBioscience Permeabilization Buffer (thermofisher scientific - ref: 00-8333-56)
Gating Strategy
Gating on lymphocytes / alive cells / CD4+ cells /
Specification
peripheral blood cells
Fixation
with the perm buffer of thermofisher (00-8333-56)

Abcam user community

Verified customer

Submitted Apr 15 2019

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (platelets)
Total protein in input
1000 µg
Immuno-precipitation step
Other - Protein G-Dynabeads
Specification
platelets

Abcam user community

Verified customer

Submitted Mar 11 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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