RecombinantRabMAb

Recombinant Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)

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Western blot - Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)
  • Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14501(B)] to NBEAL2 - N-terminal
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal
    See all NBEAL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14501(B)] to NBEAL2 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human platelet lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 303 kDa (predicted molecular weight: 303 kDa).
Notes
WB
1/1000. Detects a band of approximately 303 kDa (predicted molecular weight: 303 kDa).

Target

  • Function

    Probably involved in thrombopoiesis. Plays a role in the development or secretion of alpha-granules, that contain several growth factors important for platelet biogenesis.

  • Tissue specificity

    Expressed in megakaryocytes.

  • Involvement in disease

    Gray platelet syndrome

  • Sequence similarities

    Belongs to the WD repeat neurobeachin family.
    Contains 1 BEACH domain.
    Contains 5 WD repeats.

  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • BDPLT4 antibody
      • GPS antibody
      • NBEAL2 antibody
      • NBEL2_HUMAN antibody
      • Neurobeachin-like protein 2 antibody
      • UNQ253/PRO290 antibody
      see all

    Images

    • Western blot - Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)
      Western blot - Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)
      Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162) at 1/1000 dilution + Human platelet lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 303 kDa

    • Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)
      Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal (ab187162)

    References (1)

    Publishing research using ab187162? Please let us know so that we can cite the reference in this datasheet.

    ab187162 has been referenced in 1 publication.

    • Tomberg K  et al. Spontaneous 8bp Deletion in Nbeal2 Recapitulates the Gray Platelet Syndrome in Mice. PLoS One 11:e0150852 (2016). WB . PubMed: 26950939

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Flow Cytometry abreview for Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal

     Poor
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (peripheral blood cells)
    Permeabilization
    Yes - eBioscience Permeabilization Buffer (thermofisher scientific - ref: 00-8333-56)
    Gating Strategy
    Gating on lymphocytes / alive cells / CD4+ cells /
    Specification
    peripheral blood cells
    Fixation
    with the perm buffer of thermofisher (00-8333-56)
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2019

    Immunoprecipitation abreview for Anti-NBEAL2 antibody [EPR14501(B)] - N-terminal

     Good
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (platelets)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Other - Protein G-Dynabeads
    Specification
    platelets
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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