Application
Flow Cytometry
Sample
Human Cell (peripheral blood cells)
Permeabilization
Yes - eBioscience Permeabilization Buffer (thermofisher scientific - ref: 00-8333-56)
Gating Strategy
Gating on lymphocytes / alive cells / CD4+ cells /
Specification
peripheral blood cells
Fixation
with the perm buffer of thermofisher (00-8333-56)
Other product details
Incubation time
30 minute(s) · Temperature: 0°C · Diluent: Perm buffer thermofisher scientific - re
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
The antibody is not recognizing the protein.
The 2nd antibody is working well as it gave good results when tested with an antibody recognizing another BEACH protein : LRBA (same protein family than NBEAL2).
Abcam response
Thank you for trying out on the flow site.We do not have this data yet. I think this data is useful for other researchers.
Abcam user community
Verified customer
Submitted Apr 15 2019