Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12397] to NBL1
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-NBL1 antibody [EPR12397]
See all NBL1 primary antibodies
DescriptionRabbit monoclonal [EPR12397] to NBL1
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NBL1 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P41271
- A431 and Human prostate lysates; A431 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174843 in the following tested applications.
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).|
- Information by UniProt
- D1S1733E antibody
- DAN antibody
- DAN domain family member 1 antibody
All lanes : Anti-NBL1 antibody [EPR12397] (ab174843) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human prostate lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Immunofluorescent analysis of A431 cells labeling NBL1 with ab174843 at 1/100 dilution (red). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized A431 cells labeling NBL1 with ab174843 at 1/10 dilution (red), or negative control rabbit IgG (green).
ab174843 has not yet been referenced specifically in any publications.