Recombinant
RabMAb

Anti-NBL1 antibody [EPR12397] (ab174843)

Overview

  • Product name
    Anti-NBL1 antibody [EPR12397]
    See all NBL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12397] to NBL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NBL1 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P41271

  • Positive control
    • A431 and Human prostate lysates; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12397
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).
  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Relevance
      NBL1 (Neuroblastoma, suppression of tumorigenicity 1) is a candidate tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. NBL1 is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. It is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • D1S1733E antibody
      • DAN antibody
      • DAN domain family member 1 antibody
      • DAND 1 antibody
      • DAND1 antibody
      • Differential screening selected gene aberrant in neuroblastoma antibody
      • MGC8972 antibody
      • NB antibody
      • NBL 1 antibody
      • Neuroblastoma candidate region suppression of tumorigenicity 1 antibody
      • Neuroblastoma suppression of tumorigenicity 1 antibody
      • Neuroblastoma suppressor of tumorigenicity 1 antibody
      • NO 3 antibody
      • NO3 antibody
      • Zinc finger protein DAN antibody
      see all

    Images

    • All lanes : Anti-NBL1 antibody [EPR12397] (ab174843) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Human prostate lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 19 kDa

    • Immunofluorescent analysis of A431 cells labeling NBL1 with ab174827 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized A431 cells labeling NBL1 with ab174843 at 1/10 dilution (red), or negative control rabbit IgG (green).

    References

    ab174843 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174843.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up