Overview

  • Product name
    NBQX, AMPA / kainate antagonist
  • Description
    AMPA / kainate antagonist
  • Biological description
    Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).
  • Purity
    > 99%
  • CAS Number
    118876-58-7
  • Chemical structure
    Chemical Structure

Properties

Images

  • A - Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom). 

    B - Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom)

    Both cells were held at −68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 µm D-AP5 and 10 µm NBQX (ab120045, traces in red). Each trace represents an average of 10–20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar: (A) 50 µm, (B) 20 µm

     

    Credit: Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955

  • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

References

This product has been referenced in:
  • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »
  • Randle JC  et al. Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution. Eur J Pharmacol 215:237-44 (1992). Read more (PubMed: 1382998) »
  • Sheardown MJ  et al. 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline: a neuroprotectant for cerebral ischemia. Science 247:571-4 (1990). Read more (PubMed: 2154034) »
See all 121 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation.

As agreed, I have issued a free of charge replacement with the order number ****.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for contacting us.

Sorry again for the wrong information provided for the reconstitution of NBQX ab120045. Iwould be pleased to send you a free of charge unit of NBQX ab120045 in compensation.In order to do so, could you please send me one of the following : the order number, the PO number, the date of purchase or at least the address of your lab?

I look forward to your reply.

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Answer

Thank you for contacting us.

I have checked with the lab, and we can confirm that this compound NBQX ab120045 is not soluble in water and is actually only soluble in DMSO to 100 mM.Thiswas a mistake in our database which has now been updated.

If you require the water soluble form of NBXQ we would recommend ab120046.

We sincerely apologize for this mistake and any inconvenience this might have caused. We thank you for bringing this to our attention.Please do not hesitate to contact us if you have further questions in this regard.

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