Overview

  • Product name

    NBQX disodium salt, AMPA / kainate antagonist
  • Description

    AMPA / kainate antagonist; water soluble
  • Biological description

    Water soluble, potent, selective and competitive AMPA / kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo.

    Also available in simple stock solutions (ab144489) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity

    > 98%
  • CAS Number

    118876-58-7
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt (ab120046), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120046 (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Jurgens CW  et al. Optogenetic stimulation of the corticothalamic pathway affects relay cells and GABAergic neurons differently in the mouse visual thalamus. PLoS One 7:e45717 (2012). Read more (PubMed: 23029198) »
  • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »
  • Randle JC  et al. Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution. Eur J Pharmacol 215:237-44 (1992). Read more (PubMed: 1382998) »
  • Sheardown MJ  et al. 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline: a neuroprotectant for cerebral ischemia. Science 247:571-4 (1990). Read more (PubMed: 2154034) »
See all 27 Publications for this product

Customer reviews and Q&As

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Abreviews
NBQX disodium salt (ab120046) is easily dissolved in 0.9% saline. Used it for in vivo experiments testing the role of AMPA receptor blockade in mediating stress susceptibility. The initial results look promising.

Abcam user community

Verified customer

Submitted Oct 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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