Product nameAnti-NBR1 antibody - C-terminal
See all NBR1 primary antibodies
DescriptionRabbit polyclonal to NBR1 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human NBR1 aa 916-966 (C terminal). The exact sequence is proprietary.
Database link: Q14596
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226360 was affinity purified using an epitope specific to NBR1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 107 kDa.|
FunctionActs probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.
Sequence similaritiesContains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger.
DomainThe OPR domain mediates interaction with SQSTM1.
Cellular localizationCytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation.
- Information by UniProt
- 1A1 3B antibody
- 1A13B antibody
- B box protein antibody
All lanes : Anti-NBR1 antibody - C-terminal (ab226360) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 107 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
NBR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226360 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226360 at 1 µg/ml.
Lane 1: ab226360 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226360 has not yet been referenced specifically in any publications.