NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
Overview
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Product name
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
See all Alkaline Phosphatase Chromogen reagents -
Tested applications
Suitable for: WB, Dot blotmore details -
General notes
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
Constituents: 0.09% NBT, 0.09% 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine salt -
Concentration information loading...
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Research areas
Associated products
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Related Products
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Unmasking reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1.
Ready to use formulation. BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
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Dot blot |
1/1.
Ready to use formulation. |
Notes |
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WB
1/1. Ready to use formulation. BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
Dot blot
1/1. Ready to use formulation. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab7468 has been referenced in 3 publications.
- Gigout A et al. Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species. Arthritis Res Ther 23:113 (2021). PubMed: 33853645
- Hassani A et al. Epstein-Barr virus is present in the brain of most cases of multiple sclerosis and may engage more than just B cells. PLoS One 13:e0192109 (2018). PubMed: 29394264
- Song JW et al. 3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli. Sci Rep 6:29406 (2016). WB . PubMed: 27406241