Overview

  • Product name

    Anti-NCAM antibody [123C3.D5 + 123A8]
    See all NCAM1 primary antibodies
  • Description

    Mouse monoclonal [123C3.D5 + 123A8] to NCAM
  • Host species

    Mouse
  • Specificity

    ab200698 reacts with an extracellular domain (close to transmembrane) of NCAM.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human NCAM. Membrane preparation of a small cell lung carcinoma.
    Database link: P13591

  • Positive control

    • Human cerebellum, pancreas, neuroblastoma; Human colon ganglion tissue.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. ab200698 recognizes two proteins of the NCAM, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). ab200698 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab200698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at RT.

ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 94 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT.

IP Use 1-2 µg for 500 µg of chromatin.

Target

  • Function

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human neuro-endocrine tumor tissue labelling NCAM with ab200698.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon ganglion tissue labeling NCAM with ab200698 at 1 µg/ml.

References

This product has been referenced in:

  • Lee JY  et al. Human parthenogenetic neural stem cell grafts promote multiple regenerative processes in a traumatic brain injury model. Theranostics 9:1029-1046 (2019). Read more (PubMed: 30867814) »
See 1 Publication for this product

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