Key features and details
- Rabbit polyclonal to NCAM
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCAM antibody
See all NCAM1 primary antibodies
DescriptionRabbit polyclonal to NCAM
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
- Human cerebral cortex tissue; U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 94 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Sequence similaritiesContains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling NCAM with ab204446 at 1/50 dilution.
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling NCAM with ab204446 at 4µg/ml (green).
Anti-NCAM antibody (ab204446) + Human cerebral cortex
Predicted band size: 94 kDa
ab204446 has not yet been referenced specifically in any publications.