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Anti-NCAM antibody [NCAM1/1496] (ab218467)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/1496] (ab218467)

    Key features and details

    • Mouse monoclonal [NCAM1/1496] to NCAM
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

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    Protein
    Product image
    Recombinant Human NCAM1 protein (ab114198)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    View more associated products

    Overview

    • Product name

      Anti-NCAM antibody [NCAM1/1496]
      See all NCAM1 primary antibodies
    • Description

      Mouse monoclonal [NCAM1/1496] to NCAM
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Recombinant full length protein corresponding to Human NCAM.
      Database link: P13591

    • Positive control

      • IHC-P: Human pancreas tissue.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.05% Sodium azide
      Constituents: 99% PBS, 0.05% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      NCAM1/1496
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Growth Cone
      • Neuroscience
      • Cell Adhesion Proteins
      • ECM Proteins
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative

    Associated products

    • Alternative Versions

      • Anti-NCAM antibody [NCAM1/1496] - BSA and Azide free (ab218925)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human NCAM1 protein (ab114198)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab218467 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Primary incubation is for 30 min at RT.

    Notes
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Primary incubation is for 30 min at RT.

    Target

    • Function

      This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
    • Sequence similarities

      Contains 2 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P13591 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4684 Human
      • Omim: 116930 Human
      • SwissProt: P13591 Human
      • Unigene: 503878 Human
      • Unigene: 733031 Human
      • Alternative names

        • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
        • antigen recognized by monoclonal antibody 5.1H11 antibody
        • CD56 antibody
        • cell adhesion molecule, neural, 1 antibody
        • MSK 39 antibody
        • MSK39 antibody
        • N-CAM-1 antibody
        • NCAM 1 antibody
        • NCAM antibody
        • NCAM C antibody
        • NCAM-1 antibody
        • NCAM1 antibody
        • NCAM1_HUMAN antibody
        • NCAMC antibody
        • Neural cell adhesion molecule 1 antibody
        • Neural cell adhesion molecule NCAM antibody
        • OTTHUMP00000235666 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/1496] (ab218467)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/1496] (ab218467)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue section labeling NCAM with ab218467 at 1 µg/mL.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab218467? Please let us know so that we can cite the reference in this datasheet.

      ab218467 has not yet been referenced specifically in any publications.

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