Anti-NCAM1 (2-amino acetoacetate replaces ) antibody [MEM-188] (ab8233)

Overview

  • Product name
    Anti-NCAM1 (2-amino acetoacetate replaces ) antibody [MEM-188]
    See all NCAM1 primary antibodies
  • Description
    Mouse monoclonal [MEM-188] to NCAM1 (2-amino acetoacetate replaces )
  • Host species
    Mouse
  • Specificity
    CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Non human primates
  • Immunogen

    Tissue/ cell preparation (Human). (KG-1 acute myelogenous leukaemia cell line).

  • Positive control
    • paraprostatic autonomic ganglion

Properties

Applications

Our Abpromise guarantee covers the use of ab8233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Use standard ABC/DAB+ staining technique.
Flow Cyt Use a concentration of 0.5 - 3 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Target

  • Function
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Images

  • ab8233 staining NCAM in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed (sections were incubated with 0.5g/L of Trypsin in PBS for 30 minutes at 25ºC prior to blocking). Samples were then blocked using 5% serum for 16 hours at 4°C, then incubated with ab8233 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal at a 1/300 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Gupta J  et al. Pharmacological inhibition of p38 MAPK reduces tumor growth in patient-derived xenografts from colon tumors. Oncotarget 6:8539-51 (2015). IHC . Read more (PubMed: 25890501) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for contacting us. Although we have tested in mouse cells for FACS, we have not tesed in live cells and cannot guarantee its use for this purpose. Please let me know if you have any further questions.

Read More

Question
Answer

Code de remise : *******
Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participer à notre offre et tester ab8233 en ICC/IF. Le code promotionnel correspondant est *******, il est valable 4 mois et pour la commande d’un autre anticorps primaire gratuit.

Vous devez dans un premier temps soumettre une Abreview avec vos résultats en ICC/IF en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab8233.

Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

Termes et Conditions : https://www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Merci de votre intérêt pour ab8233.

Comme expliqué par téléphone, cet anticorps n’a pas encore été testé en ICC/IF et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab8233 en ICC/IF, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un anticorps primaire de votre choix vous sera offert.

Etapes à suivre :

1. Nous confirmer que vous souhaiteriez tester ab8233 en ICC/IF afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab8233

2. Acheter ab8233 par téléphone, fax ou internet (https://www.abcam.com)

3. Le tester en ICC/IF

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab8233 ne pas fonctionne pas en ICC/IF, après soumission de votre Abreview, vous recevrez quand même gratuitement un anticorps primaire de votre choix.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : https://www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Axillary Lymph node)
Specification
Axillary Lymph node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 28 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 28 2011

Question
Answer

Thank you for your enquiry and patience. Looking through the NCAM antibodies that we currently have, it does not appear that there is one that specifically reacts with the 180 kDa isoform and is known not to cross-react with other isoforms. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up