Overview

  • Product name

  • Description

    Goat polyclonal to NCAM1
  • Host species

    Goat
  • Specificity

    ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    KASWTRPEKQETLD

    with a Cysteine residue linker, corresponding to internal sequence amino acids 353-366 of Human NCAM (NP_851996.2).

  • Positive control

    • WB: Human brain frontal cortex lysates; IHC: human adrenal gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab118291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 94 kDa). The observed molecular weight corresponds to the glycosylated form.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Images

  • Anti-NCAM1 antibody (ab118291) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?



    The observed molecular weight corresponds to the glycosylated form of the protein.
  • Immunohistochemical analysis of paraffin embedded human adrenal gland staining NCAM1 with ab118291 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.

References

ab118291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Human Tissue sections (mouse brain)
Specification
mouse brain

Abcam user community

Verified customer

Submitted Jan 19 2016

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain

Abcam user community

Verified customer

Submitted Dec 18 2015

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